Human Malaria Parasites in Continuous Culture

Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus...

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Bibliographic Details
Published in:The Journal of parasitology 2005-06, Vol.91 (3), p.484-486
Main Authors: Trager, William, Jensen, James B
Format: Article
Language:English
Subjects:
Blood
Butchering
Carbon dioxide
Chemical suspensions
Continuous culture
Culture
DEFINING THE FIELD
Erythrocytes
Flasks
Malaria
Merozoites
Parasites
Parasitism
Parasitology
Plasmodium falciparum
Vector-borne diseases
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Description
Summary:Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. They have remained infective to Aotus.
ISSN:0022-3395
1937-2345
DOI:10.1645/0022-3395(2005)091[0484:HMPICC]2.0.CO;2