Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae

Patchoulol is a natural sesquiterpene, which is widely used in perfumes and cosmetics. In the work, the mitochondria of S. cerevisiae were engineered for patchoulol production. The patchoulol titer of mitochondria-compartmentalized strain (1.79 mg/L) was 2.71-fold higher than that of control strain...

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Bibliographic Details
Published in:Biotechnology letters 2022-04, Vol.44 (4), p.571-580
Main Authors: Tao, Xin-Yi, Lin, Yang-Chen, Wang, Feng-Qing, Liu, Qing-Hai, Ma, Yu-Shu, Liu, Min, Wei, Dong-Zhi
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Compartmentation
Cosmetics
Genomes
Life Sciences
Metabolic Engineering - methods
Microbiology
Mitochondria
Mitochondria - metabolism
Original Research Paper
Patchoulol
Perfumes
Precursors
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Sesquiterpenes - metabolism
Yeast
Yeasts
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Summary:Patchoulol is a natural sesquiterpene, which is widely used in perfumes and cosmetics. In the work, the mitochondria of S. cerevisiae were engineered for patchoulol production. The patchoulol titer of mitochondria-compartmentalized strain (1.79 mg/L) was 2.71-fold higher than that of control strain (0.66 mg/L) using genome-integrated patchoulol synthase, indicating that mitochondria compartmentation resulted in higher concentration of FPP (farnesyl pyrophosphate) precursor for patchoulol production. Moreover, when fused FPP synthase and patchoulol synthase was overexpressed in the strain with a mitochondria-localized DMAPP (dimethylallyl diphosphate) pathway, the production of patchoulol increased significantly to 19.24 mg/L, indicating more precursors were provided for patchoulol production. Nevertheless, the introduction of excess foreign proteins into mitochondria might cause a certain stress on mitochondria and showed a negative effect on the growth of yeast cells, which could hinder the expression of foreign pathways and reduce the patchoulol production. In conclusion, mitochondria-engineered yeast cells showed important potential for the enhanced biosynthesis of patchoulol, and further engineering could be considered based on the present work.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-022-03240-3