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Combination of High Solid Load, On-site Enzyme Cocktails and Surfactant in the hydrolysis of Hydrothermally Pretreated Sugarcane Bagasse and Ethanol Production

In this study, the combined strategy of using high solid load, on-site enzyme cocktails and surfactant was evaluated in saccharifications of hydrothermally pretreated sugarcane bagasse (HP-SB) and ethanol production. The hydrolyses were carried in fed-batch mode with a solid load of 10–40% (w/v) at...

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Published in:Waste and biomass valorization 2022-06, Vol.13 (6), p.3085-3094
Main Authors: de Oliveira Rodrigues, Patrísia, Moreira, Felipe Santos, Cardoso, Vicelma Luiz, Santos, Líbia Diniz, Gurgel, Leandro Vinícius Alves, Pasquini, Daniel, Baffi, Milla Alves
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cited_by cdi_FETCH-LOGICAL-c2341-d3b084d85d412a114e12fee1ca06570de859537f995df9a513e8a3203a9e18203
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creator de Oliveira Rodrigues, Patrísia
Moreira, Felipe Santos
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Baffi, Milla Alves
description In this study, the combined strategy of using high solid load, on-site enzyme cocktails and surfactant was evaluated in saccharifications of hydrothermally pretreated sugarcane bagasse (HP-SB) and ethanol production. The hydrolyses were carried in fed-batch mode with a solid load of 10–40% (w/v) at time intervals of 12 h, using two homemade enzyme extracts (ES1 from Aspergillus niger monoculture and ES2 from A. niger , Trametes versicolor and Pleurotus ostreatus consortium), 10 FPU/gds of cellulase loading at 50 °C for 72 h. After optimization of solid loading, new saccharifications were performed with the addition of 5% (w/v) surfactant (Triton X-100). The HP of SB led to a significant reduction of 69.26% in hemicelluloses content, but also preserved the cellulose fraction in HP-SB. The increase of HP-SB load in hydrolysis from 10 to 35% significantly improved the release of total reducing sugars (TRS), with an increase of 188.54% in ES1 and 177.46% in ES2. The use of Triton X-100 in saccharifications of HP-SB (30% w/v) also positively contributed to TRS production, with an increase in TRS of 6.22% in ES1 S and 24% in ES2 S . The fermentation of the hydrolysate after surfactant-assisted hydrolysis of HP-SB (30% w/v) led to an ethanol yield of 81.70% for F1 S and 88.03% for F2 S . Results demonstrated that the integrated use of high solid load, low-cost on-site enzyme cocktail and surfactant (Triton X-100) can be a promising approach to improve the efficiency of bioconversion of lignocellulosic biomass to fermentable sugars. Graphical Abstract
doi_str_mv 10.1007/s12649-022-01685-1
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1877-265X
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subjects Bagasse
Batch culture
Bioconversion
Cellulase
Cellulose
Engineering
Environment
Environmental Engineering/Biotechnology
Enzymes
Ethanol
Fermentation
Hemicellulose
Hydrolysates
Hydrolysis
Industrial Pollution Prevention
Lignocellulose
Monoculture
Onsite
Optimization
Original Paper
Renewable and Green Energy
Sugar
Sugarcane
Surfactants
Waste Management/Waste Technology
title Combination of High Solid Load, On-site Enzyme Cocktails and Surfactant in the hydrolysis of Hydrothermally Pretreated Sugarcane Bagasse and Ethanol Production
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