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Combination of High Solid Load, On-site Enzyme Cocktails and Surfactant in the hydrolysis of Hydrothermally Pretreated Sugarcane Bagasse and Ethanol Production
In this study, the combined strategy of using high solid load, on-site enzyme cocktails and surfactant was evaluated in saccharifications of hydrothermally pretreated sugarcane bagasse (HP-SB) and ethanol production. The hydrolyses were carried in fed-batch mode with a solid load of 10–40% (w/v) at...
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Published in: | Waste and biomass valorization 2022-06, Vol.13 (6), p.3085-3094 |
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creator | de Oliveira Rodrigues, Patrísia Moreira, Felipe Santos Cardoso, Vicelma Luiz Santos, Líbia Diniz Gurgel, Leandro Vinícius Alves Pasquini, Daniel Baffi, Milla Alves |
description | In this study, the combined strategy of using high solid load, on-site enzyme cocktails and surfactant was evaluated in saccharifications of hydrothermally pretreated sugarcane bagasse (HP-SB) and ethanol production. The hydrolyses were carried in fed-batch mode with a solid load of 10–40% (w/v) at time intervals of 12 h, using two homemade enzyme extracts (ES1 from
Aspergillus niger
monoculture and ES2 from
A. niger
,
Trametes versicolor
and
Pleurotus ostreatus
consortium), 10 FPU/gds of cellulase loading at 50 °C for 72 h. After optimization of solid loading, new saccharifications were performed with the addition of 5% (w/v) surfactant (Triton X-100). The HP of SB led to a significant reduction of 69.26% in hemicelluloses content, but also preserved the cellulose fraction in HP-SB. The increase of HP-SB load in hydrolysis from 10 to 35% significantly improved the release of total reducing sugars (TRS), with an increase of 188.54% in ES1 and 177.46% in ES2. The use of Triton X-100 in saccharifications of HP-SB (30% w/v) also positively contributed to TRS production, with an increase in TRS of 6.22% in ES1
S
and 24% in ES2
S
. The fermentation of the hydrolysate after surfactant-assisted hydrolysis of HP-SB (30% w/v) led to an ethanol yield of 81.70% for F1
S
and 88.03% for F2
S
. Results demonstrated that the integrated use of high solid load, low-cost on-site enzyme cocktail and surfactant (Triton X-100) can be a promising approach to improve the efficiency of bioconversion of lignocellulosic biomass to fermentable sugars.
Graphical Abstract |
doi_str_mv | 10.1007/s12649-022-01685-1 |
format | article |
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Aspergillus niger
monoculture and ES2 from
A. niger
,
Trametes versicolor
and
Pleurotus ostreatus
consortium), 10 FPU/gds of cellulase loading at 50 °C for 72 h. After optimization of solid loading, new saccharifications were performed with the addition of 5% (w/v) surfactant (Triton X-100). The HP of SB led to a significant reduction of 69.26% in hemicelluloses content, but also preserved the cellulose fraction in HP-SB. The increase of HP-SB load in hydrolysis from 10 to 35% significantly improved the release of total reducing sugars (TRS), with an increase of 188.54% in ES1 and 177.46% in ES2. The use of Triton X-100 in saccharifications of HP-SB (30% w/v) also positively contributed to TRS production, with an increase in TRS of 6.22% in ES1
S
and 24% in ES2
S
. The fermentation of the hydrolysate after surfactant-assisted hydrolysis of HP-SB (30% w/v) led to an ethanol yield of 81.70% for F1
S
and 88.03% for F2
S
. Results demonstrated that the integrated use of high solid load, low-cost on-site enzyme cocktail and surfactant (Triton X-100) can be a promising approach to improve the efficiency of bioconversion of lignocellulosic biomass to fermentable sugars.
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Aspergillus niger
monoculture and ES2 from
A. niger
,
Trametes versicolor
and
Pleurotus ostreatus
consortium), 10 FPU/gds of cellulase loading at 50 °C for 72 h. After optimization of solid loading, new saccharifications were performed with the addition of 5% (w/v) surfactant (Triton X-100). The HP of SB led to a significant reduction of 69.26% in hemicelluloses content, but also preserved the cellulose fraction in HP-SB. The increase of HP-SB load in hydrolysis from 10 to 35% significantly improved the release of total reducing sugars (TRS), with an increase of 188.54% in ES1 and 177.46% in ES2. The use of Triton X-100 in saccharifications of HP-SB (30% w/v) also positively contributed to TRS production, with an increase in TRS of 6.22% in ES1
S
and 24% in ES2
S
. The fermentation of the hydrolysate after surfactant-assisted hydrolysis of HP-SB (30% w/v) led to an ethanol yield of 81.70% for F1
S
and 88.03% for F2
S
. Results demonstrated that the integrated use of high solid load, low-cost on-site enzyme cocktail and surfactant (Triton X-100) can be a promising approach to improve the efficiency of bioconversion of lignocellulosic biomass to fermentable sugars.
Graphical Abstract</description><subject>Bagasse</subject><subject>Batch culture</subject><subject>Bioconversion</subject><subject>Cellulase</subject><subject>Cellulose</subject><subject>Engineering</subject><subject>Environment</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>Fermentation</subject><subject>Hemicellulose</subject><subject>Hydrolysates</subject><subject>Hydrolysis</subject><subject>Industrial Pollution Prevention</subject><subject>Lignocellulose</subject><subject>Monoculture</subject><subject>Onsite</subject><subject>Optimization</subject><subject>Original Paper</subject><subject>Renewable and Green Energy</subject><subject>Sugar</subject><subject>Sugarcane</subject><subject>Surfactants</subject><subject>Waste Management/Waste Technology</subject><issn>1877-2641</issn><issn>1877-265X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kc1KLDEQhRu5gqK-gKuAW_uaSvp36R1GRxhQUMFdKDvVM9GexJtkFu3L-KpmZkR3rqoqnPNVqJNlp8D_Auf1RQBRFW3Ohcg5VE2Zw152CE1d56Iqn_589wUcZCchvHDOBUAjZH2YfUzc6tlYjMZZ5no2M4slu3eD0WzuUJ-zW5sHE4lN7fu4IjZx3WtEMwSGVrP7te-xi2gjM5bFJbHlqL0bxmDClraZ0rNf4TCM7M5T9ISRNs4F-g4tsX-4wBBoy5vGJVo3JKHT627zp-Nsv8ch0MlXPcoer6YPk1k-v72-mVzO807IAnItn3lT6KbUBQgEKAhETwQd8qqsuaambEtZ921b6r7FEiQ1KAWX2FK6BJdH2dmO--bd_zWFqF7c2tu0UomqAgCeoEkldqrOuxA89erNmxX6UQFXmyzULguVslDbLBQkk9yZQhLbBfkf9C-uTz-djbE</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>de Oliveira Rodrigues, Patrísia</creator><creator>Moreira, Felipe Santos</creator><creator>Cardoso, Vicelma Luiz</creator><creator>Santos, Líbia Diniz</creator><creator>Gurgel, Leandro Vinícius Alves</creator><creator>Pasquini, Daniel</creator><creator>Baffi, Milla Alves</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-8098-1387</orcidid><orcidid>https://orcid.org/0000-0002-7128-5280</orcidid><orcidid>https://orcid.org/0000-0003-0983-889X</orcidid><orcidid>https://orcid.org/0000-0002-3052-7503</orcidid><orcidid>https://orcid.org/0000-0002-8278-6988</orcidid><orcidid>https://orcid.org/0000-0001-5249-8670</orcidid><orcidid>https://orcid.org/0000-0001-7441-5143</orcidid></search><sort><creationdate>20220601</creationdate><title>Combination of High Solid Load, On-site Enzyme Cocktails and Surfactant in the hydrolysis of Hydrothermally Pretreated Sugarcane Bagasse and Ethanol Production</title><author>de Oliveira Rodrigues, Patrísia ; Moreira, Felipe Santos ; Cardoso, Vicelma Luiz ; Santos, Líbia Diniz ; Gurgel, Leandro Vinícius Alves ; Pasquini, Daniel ; Baffi, Milla Alves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2341-d3b084d85d412a114e12fee1ca06570de859537f995df9a513e8a3203a9e18203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bagasse</topic><topic>Batch culture</topic><topic>Bioconversion</topic><topic>Cellulase</topic><topic>Cellulose</topic><topic>Engineering</topic><topic>Environment</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>Fermentation</topic><topic>Hemicellulose</topic><topic>Hydrolysates</topic><topic>Hydrolysis</topic><topic>Industrial Pollution Prevention</topic><topic>Lignocellulose</topic><topic>Monoculture</topic><topic>Onsite</topic><topic>Optimization</topic><topic>Original Paper</topic><topic>Renewable and Green Energy</topic><topic>Sugar</topic><topic>Sugarcane</topic><topic>Surfactants</topic><topic>Waste Management/Waste Technology</topic><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira Rodrigues, Patrísia</creatorcontrib><creatorcontrib>Moreira, Felipe Santos</creatorcontrib><creatorcontrib>Cardoso, Vicelma Luiz</creatorcontrib><creatorcontrib>Santos, Líbia Diniz</creatorcontrib><creatorcontrib>Gurgel, Leandro Vinícius Alves</creatorcontrib><creatorcontrib>Pasquini, Daniel</creatorcontrib><creatorcontrib>Baffi, Milla Alves</creatorcontrib><collection>CrossRef</collection><jtitle>Waste and biomass valorization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira Rodrigues, Patrísia</au><au>Moreira, Felipe Santos</au><au>Cardoso, Vicelma Luiz</au><au>Santos, Líbia Diniz</au><au>Gurgel, Leandro Vinícius Alves</au><au>Pasquini, Daniel</au><au>Baffi, Milla Alves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combination of High Solid Load, On-site Enzyme Cocktails and Surfactant in the hydrolysis of Hydrothermally Pretreated Sugarcane Bagasse and Ethanol Production</atitle><jtitle>Waste and biomass valorization</jtitle><stitle>Waste Biomass Valor</stitle><date>2022-06-01</date><risdate>2022</risdate><volume>13</volume><issue>6</issue><spage>3085</spage><epage>3094</epage><pages>3085-3094</pages><issn>1877-2641</issn><eissn>1877-265X</eissn><abstract>In this study, the combined strategy of using high solid load, on-site enzyme cocktails and surfactant was evaluated in saccharifications of hydrothermally pretreated sugarcane bagasse (HP-SB) and ethanol production. The hydrolyses were carried in fed-batch mode with a solid load of 10–40% (w/v) at time intervals of 12 h, using two homemade enzyme extracts (ES1 from
Aspergillus niger
monoculture and ES2 from
A. niger
,
Trametes versicolor
and
Pleurotus ostreatus
consortium), 10 FPU/gds of cellulase loading at 50 °C for 72 h. After optimization of solid loading, new saccharifications were performed with the addition of 5% (w/v) surfactant (Triton X-100). The HP of SB led to a significant reduction of 69.26% in hemicelluloses content, but also preserved the cellulose fraction in HP-SB. The increase of HP-SB load in hydrolysis from 10 to 35% significantly improved the release of total reducing sugars (TRS), with an increase of 188.54% in ES1 and 177.46% in ES2. The use of Triton X-100 in saccharifications of HP-SB (30% w/v) also positively contributed to TRS production, with an increase in TRS of 6.22% in ES1
S
and 24% in ES2
S
. The fermentation of the hydrolysate after surfactant-assisted hydrolysis of HP-SB (30% w/v) led to an ethanol yield of 81.70% for F1
S
and 88.03% for F2
S
. Results demonstrated that the integrated use of high solid load, low-cost on-site enzyme cocktail and surfactant (Triton X-100) can be a promising approach to improve the efficiency of bioconversion of lignocellulosic biomass to fermentable sugars.
Graphical Abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12649-022-01685-1</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-8098-1387</orcidid><orcidid>https://orcid.org/0000-0002-7128-5280</orcidid><orcidid>https://orcid.org/0000-0003-0983-889X</orcidid><orcidid>https://orcid.org/0000-0002-3052-7503</orcidid><orcidid>https://orcid.org/0000-0002-8278-6988</orcidid><orcidid>https://orcid.org/0000-0001-5249-8670</orcidid><orcidid>https://orcid.org/0000-0001-7441-5143</orcidid></addata></record> |
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subjects | Bagasse Batch culture Bioconversion Cellulase Cellulose Engineering Environment Environmental Engineering/Biotechnology Enzymes Ethanol Fermentation Hemicellulose Hydrolysates Hydrolysis Industrial Pollution Prevention Lignocellulose Monoculture Onsite Optimization Original Paper Renewable and Green Energy Sugar Sugarcane Surfactants Waste Management/Waste Technology |
title | Combination of High Solid Load, On-site Enzyme Cocktails and Surfactant in the hydrolysis of Hydrothermally Pretreated Sugarcane Bagasse and Ethanol Production |
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