Development of 105 SNP markers in endangered turtle species Pelodiscus sinensis using RAD-seq

Restriction site-associated DNA sequencing (RAD-seq) was used to develop single nucleotide polymorphisms (SNP) marker for the Pelodiscus sinensis , which is a commercially important species in China. A total of 105 high-quality SNP markers were identified. The genetic statistics showed that the mino...

Full description

Saved in:
Bibliographic Details
Published in:Conservation genetics resources 2022-03, Vol.14 (1), p.27-30
Main Authors: Xie, Qiming, Liu, Fan, Zhang, Jilong, Li, Xilei, Chen, Tiantian, Fang, Guoxia, Ma, Rensheng, Su, Shiping
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
DNA sequencing
Ecology
Endangered species
Evolutionary Biology
Gene frequency
Genetics
Genomes
Heterozygosity
Life Sciences
Mitochondrial DNA
Pelodiscus sinensis
Plant Genetics and Genomics
Population genetics
Population studies
Single-nucleotide polymorphism
Technical Note
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Restriction site-associated DNA sequencing (RAD-seq) was used to develop single nucleotide polymorphisms (SNP) marker for the Pelodiscus sinensis , which is a commercially important species in China. A total of 105 high-quality SNP markers were identified. The genetic statistics showed that the minor allele frequency varied from 0.0571 to 0.3382 and the observed heterozygosity and the expected heterozygosity ranged from 0.1143 to 0.6765 and 0.1078 to 0.4477, respectively. These novel SNP markers will serve as a valuable genetic tool for future conservation and population genetics studies for P. sinensis .
ISSN:1877-7260
1877-7252
1877-7260
DOI:10.1007/s12686-021-01238-z