Assessment of the Diagnostic Performance of Normal and Irradiated Schistosoma mansoni Cercarial Antigens using ELISA

ABSTRACT Irradiation of Schistosoma mansoni cercariae induces structural and motor changes in its surface molecules converting it from poor immunogenic to highly immunogenic surface. This study aims to comparatively analyze normal and gamma radiation-attenuated Schistosoma mansoni cercarial antigens...

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Bibliographic Details
Published in:Pakistan journal of zoology 2022-02, Vol.54 (1), p.1
Main Authors: El-Derbawy, Mona Mohammed, Hafez, Eman Naser, Daym Abd Rabbo, Mona Abdel, Toaleb, Nagwa Ibrahim, Sayed El-Ahl, Saedia Abdel Hady, El-Aswad, Bahaa Eldkeen Wade
Format: Article
Language:English
Subjects:
Accuracy
Antibodies
Antigens
Binding sites
Cross-reactivity
Diagnosis
Enzyme-linked immunosorbent assay
Enzymes
Gamma rays
Immune system
Immunogenicity
Immunoglobulin G
Infections
Irradiation
Laboratory animals
Parasites
Parasitic diseases
Radiation
Schistosoma mansoni
Schistosomiasis
Serology
Testing
Toxoplasmosis
Tropical diseases
Variance analysis
γ Radiation
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Summary:ABSTRACT Irradiation of Schistosoma mansoni cercariae induces structural and motor changes in its surface molecules converting it from poor immunogenic to highly immunogenic surface. This study aims to comparatively analyze normal and gamma radiation-attenuated Schistosoma mansoni cercarial antigens (SCA) by ELISA for serodiagnosis. Total IgG ELISA was assessed to evaluate the potency of two parts of Schistosoma mansoni ceracrial antigens (2000 each) one normal and other exposed to attenuating dose of 400 Gy gamma radiations. The study was conducted on fifty two individual sera samples divided as follows: Group included 21 obtained from patients who were positive for schistosomiasis and negative for other parasitic infections; Group included 18 sera from patients who were negative for schistosomiasis and positive for other parasitic infections; (3) fascioliasis, (5) amoebiasis, (7) toxoplasmosis and (3) hydatidosis and Group included (13) sera from healthy individuals all were diagnosed parasitologically and serologically. Although both antigens achieved 100 % sensitivity and negative predictive value (NPP), irradiated SCA revealed better specificity, accuracy and positive predictive value (PPV) of 87 %, 92.3% and 84 %, respectively. While normal SCA revealed 83.9%, 90.4 % and 80.7 %, respectively. It was concluded that irradiated SCA achieved better remarkable ODs than normal SCA and better specificity due to low cross-reactivity with other parasites. S0 it can be used as a tool for diagnosis of chronic schistosomiasis.
ISSN:0030-9923
DOI:10.17582/journal.pjz/20190301060337