Hormonal and histological studies related to in vitro banana bud formation

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2000-01, Vol.63 (3), p.187-192
• Main Authors: ZAFFARI, Gilmar R, KERBAUY, Gilberto B, KRAUS, Jane E, ROMANO, Elaine C
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Colorimetry; Cytokinins; Economic plant physiology; Eukaryotic cell cultures; Explants; Fundamental and applied biological sciences. Psychology; Growth and development; High-performance liquid chromatography; In vitro propagation: entire plant regeneration from tissues and cell cultures; Inoculation; Liquid chromatography; Methods. Procedures. Technologies; Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence; Plant cells and fungal cells; Subgroups
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1023/A:1010611318932

Shoot apices of Musa subgroup AAA `Grande Naine' were used for in vitro culture establishment. The endogenous hormone levels and their effects on bud formation were evaluated during a 75-day period. Cytokinins, IAA and ABA were separated by HPLC and quantified by means of ELISA. Enzymatic degradation of IAA was determined by the colorimetric method. Explants were maintained on establishment medium for 60 days. The endogenous cytokinins were higher in the basal portion of the explant. Subculture to proliferation medium (65 to 75 days) resulted in a substantial increase of cytokinins in the basal portion and in a decline in the apical portion. 2iP was the predominant cytokinin in the tissue. The endogenous level of IAA and the IAA/cytokinin ratio decreased after the 65th day of culture. The level of ABA was reduced from the time of inoculation up to the 75th day of culture. Histological analysis indicated that buds formed at the leaf base at the 65th day of culture.