First report of association of Ca. Phytoplasma australasia‐related strain (16SrII‐D) with Thuja occidentalis plants showing leaf yellowing symptoms in Uttarakhand province, India

Thuja occidentalis L. is an evergreen coniferous species of high medicinal value with fan‐like branches and scaly leaves. Leaf yellowing symptoms were recorded on T. occidentalis plants in 2019 at Mukteshwar, Uttarakhand, India. Using the universal 16S rRNA and secA gene phytoplasma‐specific primers...

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Bibliographic Details
Published in:Journal of phytopathology 2022-06, Vol.170 (6), p.359-362
Main Authors: Mitra, Surabhi, Reddy, Madem G., Rao, Govind P.
Format: Article
Language:English
Subjects:
16S rRNA gene
16SrII‐D subgroup
In silico RFLP
Leaves
Phytoplasma
Plants
rRNA 16S
SecA gene
Signs and symptoms
Subgroups
Thuja occidentalis
White cedar
Yellowing
Yellows
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Summary:Thuja occidentalis L. is an evergreen coniferous species of high medicinal value with fan‐like branches and scaly leaves. Leaf yellowing symptoms were recorded on T. occidentalis plants in 2019 at Mukteshwar, Uttarakhand, India. Using the universal 16S rRNA and secA gene phytoplasma‐specific primers, the expected amplicons of ~1.25 kb and 480 bp were consistently obtained in ten symptomatic thuja leaf samples. Virtual RFLP profiles showed that the thuja phytoplasma strains were associated with Ca. Phytoplasma australasia (16SrII‐D subgroup)‐related strain by matching the restriction patterns of the 16S rRNA gene. This is the first time a phytoplasma from the 16SrII‐D subgroup has been recorded with thuja yellows disease in the world.
ISSN:0931-1785
1439-0434
DOI:10.1111/jph.13090