Microcalorimetric investigation of the effect of La3+ on mitochondria isolated from avian chicken liver tissue cells

A microcalorimetric technique was used to evaluate the influence of La3+ on mitochondria isolated from the liver tissue of Avian chicken. By means of LKB-2277 bioactivity monitor, ampoule method at 37°C, we obtained the thermogenic curves of the metabolism of mitochondria. After isolation from the c...

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Bibliographic Details
Published in:Journal of thermal analysis and calorimetry 2003, Vol.73 (3), p.843-849
Main Authors: PENG, L, YI, L, JIA, H, FENGJIAO, D, DAIWEN, P, SONGSHENG, Q
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects
Liver
Maximum power
Metabolism
Mitochondria
Nutrients
Rate constants
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:A microcalorimetric technique was used to evaluate the influence of La3+ on mitochondria isolated from the liver tissue of Avian chicken. By means of LKB-2277 bioactivity monitor, ampoule method at 37°C, we obtained the thermogenic curves of the metabolism of mitochondria. After isolation from the chicken liver tissue, mitochondria still have metabolic activity and can live for a long time depending on the stored nutrients. In order to analyze the results, the maximum power (Pm) and the decline rate constants (kd) were obtained. The addition of La3+ results in an increase of the maximum heat production and decline rate constants. Furthermore, values of Pm and kd are linked to the concentration of La3+. According to the thermogenic curves under different conditions, it is clear that metabolic mechanism of mitochondria has been changed with the addition of La3+.
ISSN:1388-6150
1588-2926
DOI:10.1023/A:1025842800225