Nutrient Limitation is the Main Regulatory Factor for Carotenoid Accumulation and for Psy and Pds Steady State Transcript Levels in Dunaliella salina (Chlorophyta) Exposed to High Light and Salt Stress

Dunaliella salina (Dunal) Teodoresco (1905) is a green unicellular alga able to withstand severe salt, light, and nutrient stress, adaptations necessary to grow in harsh environments such as salt ponds. In response to such growth conditions, this microalga accumulates high amounts of β-carotene in i...

Full description

Saved in:
Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2008-09, Vol.10 (5), p.602-611, Article 602
Main Authors: Coesel, Sacha Nicole, Baumgartner, Alexandra Cordeiro, Teles, Licia Marlene, Ramos, Ana Alexandra, Henriques, Nuno Miguel, Cancela, Leonor, Varela, João Carlos Serafim
Format: Article
Language:English
Subjects:
Abiotic stress
Accumulation
Adaptation
Alkyl and Aryl Transferases - genetics
Biomedical and Life Sciences
Biosynthesis
Carotene
Carotenoid biosynthesis
Carotenoids
Carotenoids - metabolism
Cell division
Cells
Chlorophyll
Chlorophyta - drug effects
Chlorophyta - genetics
Chlorophyta - metabolism
Chlorophyta - radiation effects
Chloroplasts
Culture Media - chemistry
Desaturase
Dunaliella salina
Engineering
Enzymes
Food
Freshwater & Marine Ecology
Gene Expression Regulation - drug effects
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
Growth conditions
Harsh environments
Life Sciences
Light
Marine biology
Microbiology
Mineral nutrients
mRNA
nitrates
Nitrates - analysis
Nutrient availability
Nutrients
Organisms
Original Article
Oxidoreductases - genetics
Phytoene synthase
Polymerase chain reaction
Regulation
RNA, Messenger - metabolism
Salinity
Salt
Salts
Sodium chloride
Sodium Chloride - pharmacology
Steady state
Stress
Studies
Time Factors
Zoology
β-Carotene
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c421t-48fed0e9b443729089f2c4306ba43e6c6973046e36754496583a73fe5df4d1643
cites cdi_FETCH-LOGICAL-c421t-48fed0e9b443729089f2c4306ba43e6c6973046e36754496583a73fe5df4d1643
container_end_page 611
container_issue 5
container_start_page 602
container_title Marine biotechnology (New York, N.Y.)
container_volume 10
creator Coesel, Sacha Nicole
Baumgartner, Alexandra Cordeiro
Teles, Licia Marlene
Ramos, Ana Alexandra
Henriques, Nuno Miguel
Cancela, Leonor
Varela, João Carlos Serafim
description Dunaliella salina (Dunal) Teodoresco (1905) is a green unicellular alga able to withstand severe salt, light, and nutrient stress, adaptations necessary to grow in harsh environments such as salt ponds. In response to such growth conditions, this microalga accumulates high amounts of β-carotene in its single chloroplast. In this study, we show that carotenoid accumulation is consistently inhibited in cells grown in nutrient-supplemented media and exposed either to high-light or medium-low-light conditions. Likewise, carotenogenesis in cells shifted to higher salinity (up to 27% NaCl) under medium-low-light conditions is inhibited by the presence of nutrients. The steady-state levels of transcripts encoding phytoene synthase and phytoene desaturase increased substantially in D. salina cells shifted to high light or high salt under nutrient-limiting conditions, whereas the presence of nutrients inhibited this response. The regulatory effect of nutrient availability on the accumulation of carotenoids and messenger RNA levels of the first two enzymes committed to carotenoid biosynthesis is discussed.
doi_str_mv 10.1007/s10126-008-9100-2
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2664666043</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1898932541</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-48fed0e9b443729089f2c4306ba43e6c6973046e36754496583a73fe5df4d1643</originalsourceid><addsrcrecordid>eNp9Uc1u1DAQjhCIlsIDcAELLnAI-G-d5FgtLUVaoGLbszWbTBJX2Ti1HUQekbfC2azoCQ7WjDzfjz1fkrxk9AOjNPvoGWVcpZTmaREvUv4oOWVSqJRzoR7_7Xl-kjzz_o5GTibo0-SE5VIWitLT5Pe3MTiDfSAbszcBgrE9MZ6EFslXMD35gc3YQbBuIpdQxkrqeNbgbMDemoqcl-W4nyEzE_rqML_206G_rjzZBoRqigUCkhsHvS-dGaIh_sTOk-jxaeyhM9h1QHxseiDv1m1nnR3aKcB7cvFrsB4rEiy5Mk0bn9q04aC_hS5EZYfeP0-e1NB5fHGsZ8nt5cXN-irdfP_8ZX2-SUvJWUhlXmNFsdhJKTJe0LyoeSkFVTuQAlWpirgiqVCobDUvaZULyESNq6qWFVNSnCVvF93B2fsRfdB3dnTxA15zpaRSikoRUW_-iYqeKssojyC2gEpnvXdY68GZPbhJM6rnhPWSsI4J6zlhPXNeHYXH3R6rB8Yx0gjgC8DHUd-ge3D-n-rrhVSD1dA44_XtllMmKC244iIXfwBdW7rP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>208967702</pqid></control><display><type>article</type><title>Nutrient Limitation is the Main Regulatory Factor for Carotenoid Accumulation and for Psy and Pds Steady State Transcript Levels in Dunaliella salina (Chlorophyta) Exposed to High Light and Salt Stress</title><source>ABI/INFORM Global</source><source>Springer Nature</source><creator>Coesel, Sacha Nicole ; Baumgartner, Alexandra Cordeiro ; Teles, Licia Marlene ; Ramos, Ana Alexandra ; Henriques, Nuno Miguel ; Cancela, Leonor ; Varela, João Carlos Serafim</creator><creatorcontrib>Coesel, Sacha Nicole ; Baumgartner, Alexandra Cordeiro ; Teles, Licia Marlene ; Ramos, Ana Alexandra ; Henriques, Nuno Miguel ; Cancela, Leonor ; Varela, João Carlos Serafim</creatorcontrib><description>Dunaliella salina (Dunal) Teodoresco (1905) is a green unicellular alga able to withstand severe salt, light, and nutrient stress, adaptations necessary to grow in harsh environments such as salt ponds. In response to such growth conditions, this microalga accumulates high amounts of β-carotene in its single chloroplast. In this study, we show that carotenoid accumulation is consistently inhibited in cells grown in nutrient-supplemented media and exposed either to high-light or medium-low-light conditions. Likewise, carotenogenesis in cells shifted to higher salinity (up to 27% NaCl) under medium-low-light conditions is inhibited by the presence of nutrients. The steady-state levels of transcripts encoding phytoene synthase and phytoene desaturase increased substantially in D. salina cells shifted to high light or high salt under nutrient-limiting conditions, whereas the presence of nutrients inhibited this response. The regulatory effect of nutrient availability on the accumulation of carotenoids and messenger RNA levels of the first two enzymes committed to carotenoid biosynthesis is discussed.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-008-9100-2</identifier><identifier>PMID: 18449600</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Abiotic stress ; Accumulation ; Adaptation ; Alkyl and Aryl Transferases - genetics ; Biomedical and Life Sciences ; Biosynthesis ; Carotene ; Carotenoid biosynthesis ; Carotenoids ; Carotenoids - metabolism ; Cell division ; Cells ; Chlorophyll ; Chlorophyta - drug effects ; Chlorophyta - genetics ; Chlorophyta - metabolism ; Chlorophyta - radiation effects ; Chloroplasts ; Culture Media - chemistry ; Desaturase ; Dunaliella salina ; Engineering ; Enzymes ; Food ; Freshwater &amp; Marine Ecology ; Gene Expression Regulation - drug effects ; Geranylgeranyl-Diphosphate Geranylgeranyltransferase ; Growth conditions ; Harsh environments ; Life Sciences ; Light ; Marine biology ; Microbiology ; Mineral nutrients ; mRNA ; nitrates ; Nitrates - analysis ; Nutrient availability ; Nutrients ; Organisms ; Original Article ; Oxidoreductases - genetics ; Phytoene synthase ; Polymerase chain reaction ; Regulation ; RNA, Messenger - metabolism ; Salinity ; Salt ; Salts ; Sodium chloride ; Sodium Chloride - pharmacology ; Steady state ; Stress ; Studies ; Time Factors ; Zoology ; β-Carotene</subject><ispartof>Marine biotechnology (New York, N.Y.), 2008-09, Vol.10 (5), p.602-611, Article 602</ispartof><rights>Springer Science+Business Media, LLC 2008</rights><rights>Springer Science+Business Media, LLC 2008.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-48fed0e9b443729089f2c4306ba43e6c6973046e36754496583a73fe5df4d1643</citedby><cites>FETCH-LOGICAL-c421t-48fed0e9b443729089f2c4306ba43e6c6973046e36754496583a73fe5df4d1643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/208967702/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/208967702?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,11688,27924,27925,36060,44363,74895</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18449600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coesel, Sacha Nicole</creatorcontrib><creatorcontrib>Baumgartner, Alexandra Cordeiro</creatorcontrib><creatorcontrib>Teles, Licia Marlene</creatorcontrib><creatorcontrib>Ramos, Ana Alexandra</creatorcontrib><creatorcontrib>Henriques, Nuno Miguel</creatorcontrib><creatorcontrib>Cancela, Leonor</creatorcontrib><creatorcontrib>Varela, João Carlos Serafim</creatorcontrib><title>Nutrient Limitation is the Main Regulatory Factor for Carotenoid Accumulation and for Psy and Pds Steady State Transcript Levels in Dunaliella salina (Chlorophyta) Exposed to High Light and Salt Stress</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Dunaliella salina (Dunal) Teodoresco (1905) is a green unicellular alga able to withstand severe salt, light, and nutrient stress, adaptations necessary to grow in harsh environments such as salt ponds. In response to such growth conditions, this microalga accumulates high amounts of β-carotene in its single chloroplast. In this study, we show that carotenoid accumulation is consistently inhibited in cells grown in nutrient-supplemented media and exposed either to high-light or medium-low-light conditions. Likewise, carotenogenesis in cells shifted to higher salinity (up to 27% NaCl) under medium-low-light conditions is inhibited by the presence of nutrients. The steady-state levels of transcripts encoding phytoene synthase and phytoene desaturase increased substantially in D. salina cells shifted to high light or high salt under nutrient-limiting conditions, whereas the presence of nutrients inhibited this response. The regulatory effect of nutrient availability on the accumulation of carotenoids and messenger RNA levels of the first two enzymes committed to carotenoid biosynthesis is discussed.</description><subject>Abiotic stress</subject><subject>Accumulation</subject><subject>Adaptation</subject><subject>Alkyl and Aryl Transferases - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Carotene</subject><subject>Carotenoid biosynthesis</subject><subject>Carotenoids</subject><subject>Carotenoids - metabolism</subject><subject>Cell division</subject><subject>Cells</subject><subject>Chlorophyll</subject><subject>Chlorophyta - drug effects</subject><subject>Chlorophyta - genetics</subject><subject>Chlorophyta - metabolism</subject><subject>Chlorophyta - radiation effects</subject><subject>Chloroplasts</subject><subject>Culture Media - chemistry</subject><subject>Desaturase</subject><subject>Dunaliella salina</subject><subject>Engineering</subject><subject>Enzymes</subject><subject>Food</subject><subject>Freshwater &amp; Marine Ecology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Geranylgeranyl-Diphosphate Geranylgeranyltransferase</subject><subject>Growth conditions</subject><subject>Harsh environments</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Marine biology</subject><subject>Microbiology</subject><subject>Mineral nutrients</subject><subject>mRNA</subject><subject>nitrates</subject><subject>Nitrates - analysis</subject><subject>Nutrient availability</subject><subject>Nutrients</subject><subject>Organisms</subject><subject>Original Article</subject><subject>Oxidoreductases - genetics</subject><subject>Phytoene synthase</subject><subject>Polymerase chain reaction</subject><subject>Regulation</subject><subject>RNA, Messenger - metabolism</subject><subject>Salinity</subject><subject>Salt</subject><subject>Salts</subject><subject>Sodium chloride</subject><subject>Sodium Chloride - pharmacology</subject><subject>Steady state</subject><subject>Stress</subject><subject>Studies</subject><subject>Time Factors</subject><subject>Zoology</subject><subject>β-Carotene</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNp9Uc1u1DAQjhCIlsIDcAELLnAI-G-d5FgtLUVaoGLbszWbTBJX2Ti1HUQekbfC2azoCQ7WjDzfjz1fkrxk9AOjNPvoGWVcpZTmaREvUv4oOWVSqJRzoR7_7Xl-kjzz_o5GTibo0-SE5VIWitLT5Pe3MTiDfSAbszcBgrE9MZ6EFslXMD35gc3YQbBuIpdQxkrqeNbgbMDemoqcl-W4nyEzE_rqML_206G_rjzZBoRqigUCkhsHvS-dGaIh_sTOk-jxaeyhM9h1QHxseiDv1m1nnR3aKcB7cvFrsB4rEiy5Mk0bn9q04aC_hS5EZYfeP0-e1NB5fHGsZ8nt5cXN-irdfP_8ZX2-SUvJWUhlXmNFsdhJKTJe0LyoeSkFVTuQAlWpirgiqVCobDUvaZULyESNq6qWFVNSnCVvF93B2fsRfdB3dnTxA15zpaRSikoRUW_-iYqeKssojyC2gEpnvXdY68GZPbhJM6rnhPWSsI4J6zlhPXNeHYXH3R6rB8Yx0gjgC8DHUd-ge3D-n-rrhVSD1dA44_XtllMmKC244iIXfwBdW7rP</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Coesel, Sacha Nicole</creator><creator>Baumgartner, Alexandra Cordeiro</creator><creator>Teles, Licia Marlene</creator><creator>Ramos, Ana Alexandra</creator><creator>Henriques, Nuno Miguel</creator><creator>Cancela, Leonor</creator><creator>Varela, João Carlos Serafim</creator><general>New York : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.F</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20080901</creationdate><title>Nutrient Limitation is the Main Regulatory Factor for Carotenoid Accumulation and for Psy and Pds Steady State Transcript Levels in Dunaliella salina (Chlorophyta) Exposed to High Light and Salt Stress</title><author>Coesel, Sacha Nicole ; Baumgartner, Alexandra Cordeiro ; Teles, Licia Marlene ; Ramos, Ana Alexandra ; Henriques, Nuno Miguel ; Cancela, Leonor ; Varela, João Carlos Serafim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-48fed0e9b443729089f2c4306ba43e6c6973046e36754496583a73fe5df4d1643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Abiotic stress</topic><topic>Accumulation</topic><topic>Adaptation</topic><topic>Alkyl and Aryl Transferases - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Carotene</topic><topic>Carotenoid biosynthesis</topic><topic>Carotenoids</topic><topic>Carotenoids - metabolism</topic><topic>Cell division</topic><topic>Cells</topic><topic>Chlorophyll</topic><topic>Chlorophyta - drug effects</topic><topic>Chlorophyta - genetics</topic><topic>Chlorophyta - metabolism</topic><topic>Chlorophyta - radiation effects</topic><topic>Chloroplasts</topic><topic>Culture Media - chemistry</topic><topic>Desaturase</topic><topic>Dunaliella salina</topic><topic>Engineering</topic><topic>Enzymes</topic><topic>Food</topic><topic>Freshwater &amp; Marine Ecology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Geranylgeranyl-Diphosphate Geranylgeranyltransferase</topic><topic>Growth conditions</topic><topic>Harsh environments</topic><topic>Life Sciences</topic><topic>Light</topic><topic>Marine biology</topic><topic>Microbiology</topic><topic>Mineral nutrients</topic><topic>mRNA</topic><topic>nitrates</topic><topic>Nitrates - analysis</topic><topic>Nutrient availability</topic><topic>Nutrients</topic><topic>Organisms</topic><topic>Original Article</topic><topic>Oxidoreductases - genetics</topic><topic>Phytoene synthase</topic><topic>Polymerase chain reaction</topic><topic>Regulation</topic><topic>RNA, Messenger - metabolism</topic><topic>Salinity</topic><topic>Salt</topic><topic>Salts</topic><topic>Sodium chloride</topic><topic>Sodium Chloride - pharmacology</topic><topic>Steady state</topic><topic>Stress</topic><topic>Studies</topic><topic>Time Factors</topic><topic>Zoology</topic><topic>β-Carotene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coesel, Sacha Nicole</creatorcontrib><creatorcontrib>Baumgartner, Alexandra Cordeiro</creatorcontrib><creatorcontrib>Teles, Licia Marlene</creatorcontrib><creatorcontrib>Ramos, Ana Alexandra</creatorcontrib><creatorcontrib>Henriques, Nuno Miguel</creatorcontrib><creatorcontrib>Cancela, Leonor</creatorcontrib><creatorcontrib>Varela, João Carlos Serafim</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Marine biotechnology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coesel, Sacha Nicole</au><au>Baumgartner, Alexandra Cordeiro</au><au>Teles, Licia Marlene</au><au>Ramos, Ana Alexandra</au><au>Henriques, Nuno Miguel</au><au>Cancela, Leonor</au><au>Varela, João Carlos Serafim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nutrient Limitation is the Main Regulatory Factor for Carotenoid Accumulation and for Psy and Pds Steady State Transcript Levels in Dunaliella salina (Chlorophyta) Exposed to High Light and Salt Stress</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>10</volume><issue>5</issue><spage>602</spage><epage>611</epage><pages>602-611</pages><artnum>602</artnum><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Dunaliella salina (Dunal) Teodoresco (1905) is a green unicellular alga able to withstand severe salt, light, and nutrient stress, adaptations necessary to grow in harsh environments such as salt ponds. In response to such growth conditions, this microalga accumulates high amounts of β-carotene in its single chloroplast. In this study, we show that carotenoid accumulation is consistently inhibited in cells grown in nutrient-supplemented media and exposed either to high-light or medium-low-light conditions. Likewise, carotenogenesis in cells shifted to higher salinity (up to 27% NaCl) under medium-low-light conditions is inhibited by the presence of nutrients. The steady-state levels of transcripts encoding phytoene synthase and phytoene desaturase increased substantially in D. salina cells shifted to high light or high salt under nutrient-limiting conditions, whereas the presence of nutrients inhibited this response. The regulatory effect of nutrient availability on the accumulation of carotenoids and messenger RNA levels of the first two enzymes committed to carotenoid biosynthesis is discussed.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><pmid>18449600</pmid><doi>10.1007/s10126-008-9100-2</doi><tpages>10</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1436-2228
ispartof Marine biotechnology (New York, N.Y.), 2008-09, Vol.10 (5), p.602-611, Article 602
issn 1436-2228
1436-2236
language eng
recordid cdi_proquest_journals_2664666043
source ABI/INFORM Global; Springer Nature
subjects Abiotic stress
Accumulation
Adaptation
Alkyl and Aryl Transferases - genetics
Biomedical and Life Sciences
Biosynthesis
Carotene
Carotenoid biosynthesis
Carotenoids
Carotenoids - metabolism
Cell division
Cells
Chlorophyll
Chlorophyta - drug effects
Chlorophyta - genetics
Chlorophyta - metabolism
Chlorophyta - radiation effects
Chloroplasts
Culture Media - chemistry
Desaturase
Dunaliella salina
Engineering
Enzymes
Food
Freshwater & Marine Ecology
Gene Expression Regulation - drug effects
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
Growth conditions
Harsh environments
Life Sciences
Light
Marine biology
Microbiology
Mineral nutrients
mRNA
nitrates
Nitrates - analysis
Nutrient availability
Nutrients
Organisms
Original Article
Oxidoreductases - genetics
Phytoene synthase
Polymerase chain reaction
Regulation
RNA, Messenger - metabolism
Salinity
Salt
Salts
Sodium chloride
Sodium Chloride - pharmacology
Steady state
Stress
Studies
Time Factors
Zoology
β-Carotene
title Nutrient Limitation is the Main Regulatory Factor for Carotenoid Accumulation and for Psy and Pds Steady State Transcript Levels in Dunaliella salina (Chlorophyta) Exposed to High Light and Salt Stress
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T04%3A41%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nutrient%20Limitation%20is%20the%20Main%20Regulatory%20Factor%20for%20Carotenoid%20Accumulation%20and%20for%20Psy%20and%20Pds%20Steady%20State%20Transcript%20Levels%20in%20Dunaliella%20salina%20(Chlorophyta)%20Exposed%20to%20High%20Light%20and%20Salt%20Stress&rft.jtitle=Marine%20biotechnology%20(New%20York,%20N.Y.)&rft.au=Coesel,%20Sacha%20Nicole&rft.date=2008-09-01&rft.volume=10&rft.issue=5&rft.spage=602&rft.epage=611&rft.pages=602-611&rft.artnum=602&rft.issn=1436-2228&rft.eissn=1436-2236&rft_id=info:doi/10.1007/s10126-008-9100-2&rft_dat=%3Cproquest_cross%3E1898932541%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c421t-48fed0e9b443729089f2c4306ba43e6c6973046e36754496583a73fe5df4d1643%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=208967702&rft_id=info:pmid/18449600&rfr_iscdi=true