Separation and characterization of peanut phospholipid molecular species using high-performance liquid chromatography and fast atom bombardment mass spectrometry

Total lipid extracts from peanut seed were separated on a silica column into a triacylglycerol fraction and a polar lipid fraction by high‐performance liquid chromatography (HPLC). The polar fraction containing the phospholipids was retained on the precolumn, and the triacylglycerol fraction was elu...

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Bibliographic Details
Published in:Journal of the American Oil Chemists' Society 1999-01, Vol.76 (1), p.49-56
Main Authors: Singleton, J.A. (NC State University, Raleigh, NC.), Ruan, M, Sanford, J.H, Haney, C.A, Stikeleather, L.F
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
ARACHIDE
ARACHIS HYPOGAEA
Biological and medical sciences
Bombardment
CACAHUETE
Chemical constitution
Chromatography
Economic plant physiology
Fast atom bombardment mass spectrometry
Food industries
FOSFOLIPIDOS
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
GRAINE
GROUNDNUTS
High performance liquid chromatography
HPLC
Lecithin
Lipids
Liquid chromatography
Mass spectrometry
Mass spectroscopy
molecular species
Peanuts
PHOSPHATIDE
Phosphatidylcholine
Phosphatidylethanolamine
Phosphatidylinositol
PHOSPHOLIPIDS
reversed‐phase HPLC
SEEDS
SEMILLA
Silica
Silicon dioxide
Species
Triglycerides
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Summary:Total lipid extracts from peanut seed were separated on a silica column into a triacylglycerol fraction and a polar lipid fraction by high‐performance liquid chromatography (HPLC). The polar fraction containing the phospholipids was retained on the precolumn, and the triacylglycerol fraction was eluted to a waste flask by a special valve arrangement. Phospholipids were eluted from the precolumn and separated into various classes on a silica analytical column. Each phospholipid class was manually collected and subsequently subjected to reversed‐phase HPLC in tandem with a fast atom bombardment mass spectrometer. Phosphatidylethanolamine was separated into five molecular species. Phosphatidylinositol and phosphatidylcholine were each separated into six molecular species.
ISSN:0003-021X
1558-9331
DOI:10.1007/s11746-999-0046-y