Characterisation of Solanum lycopersicoides Dun. root primordia culture with plant recovery

A liquid meristematic root primordia culture (RPC) of Solanum lycopersicoides Dun. based on persistent rhizogenesis in a modified Murashige and Skoog (1962) medium supplemented with NAA (15 mg·l−1) or 2,4-D (1 mg·l−1) was described. The meristematic clumps (2–3 mm in diameter) originating from NAA s...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2003-01, Vol.25 (4), p.385-394
Main Authors: Burza, Wojciech, Dziadczyk, Ewa, Tylicki, Adam, Kuraś, Mieczysław, Malepszy, Stefan
Format: Article
Language:English
Subjects:
2,4-D
Ammonium nitrate
Callus
Clumps
Explants
Growth regulators
Plantlets
Ploidy
Primordia
Regeneration
Seedlings
Solanum lycopersicoides
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Summary:A liquid meristematic root primordia culture (RPC) of Solanum lycopersicoides Dun. based on persistent rhizogenesis in a modified Murashige and Skoog (1962) medium supplemented with NAA (15 mg·l−1) or 2,4-D (1 mg·l−1) was described. The meristematic clumps (2–3 mm in diameter) originating from NAA supplemented medium were capable of regenerating plants through the callus stage (up to 70 %). Efficient direct plant regeneration (up to 21 %) was possible from numerous single globular-shaped root primordia (RP) structures liberated from the parental aggregates in 2,4-D supplemented proliferation medium without NH4NO3 and with a 2.5 fold increase in KNO3. The RP converted into plantlets (artificial seedlings) on solid or liquid media without growth growth regulators through the unipolar followed by the mace-shaped bipolar structure stages. The use of apical shoot bud, root apices or root segments as a primary explants brought about RPC induction and plant regeneration. The plants derived from 2 years old culture were phenotypically identical to their parental S. lycopersicoides plants and possessed the same ploidy.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-003-0020-5