Loading…

Screening for genetic elements involved in the nonhost response of sugar beet [Beta vulgaris] to the plasmodiophorid cereal root parasite Polymyxa graminis by representational difference analysis

Representational difference analysis (RDA) was used to select and clone cDNA fragments of genes whose steady state transcription was upregulated in sugar beet challenged with the nonhost parasite Polymyxa graminis. In silico analysis revealed that sequences with similarities to plant defence genes a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2007-08, Vol.73 (4), p.260-265
Main Authors: McGrann, G.R.D.(Broom's Barn Research Station, Suffolk (UK)), Martin, L.D, Kingsnorth, C.S, Asher, M.J.C, Adams, M.J, Mutasa Goettgens, E.S
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Representational difference analysis (RDA) was used to select and clone cDNA fragments of genes whose steady state transcription was upregulated in sugar beet challenged with the nonhost parasite Polymyxa graminis. In silico analysis revealed that sequences with similarities to plant defence genes as well as genes of unknown function were represented amongst the cloned cDNAs. The utility of RDA was verified when, in material from the nonhost interaction, semiquantitative RT-PCR confirmed transcriptional upregulation of at least 10 of 17 genes randomly selected from the RDA library. Time-course transcriptional analysis of two plant defence gene-like sequences demonstrated that, in sugar beet, both were upregulated within 1 h in response to P. graminis but not to P. betae. This work comprises the first report of an active response by sugar beet to P. graminis.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-007-0024-x