An Exo III-assisted catalytic hairpin assembly-based self-fluorescence aptasensor for pesticide detection

This study proposed a self-fluorescence aptasensor (SFA) based on an Exo III-assisted catalytic hairpin assembly (EACHA) for pesticide detection. The aptamer in this sensor can specifically recognize the target due to the favorable biological binding affinity and also can drive the EACHA by modifyin...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2022-05, Vol.358, p.131441, Article 131441
Main Authors: Xu, Yuancong, Cheng, Nan, Luo, Yunbo, Huang, Kunlun, Chang, Qiaoying, Pang, Guofang, Xu, Wentao
Format: Article
Language:English
Subjects:
2-Aminopurine
Aptasensor
Assembly
Chlorpyrifos
Exo III-assisted catalytic hairpin assembly
Fluorescence
Pesticide detection
Pesticides
Sensitivity analysis
Target detection
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Summary:This study proposed a self-fluorescence aptasensor (SFA) based on an Exo III-assisted catalytic hairpin assembly (EACHA) for pesticide detection. The aptamer in this sensor can specifically recognize the target due to the favorable biological binding affinity and also can drive the EACHA by modifying the spatial configuration. EACHA was designed as a cyclic amplification process that improved the rate of reused aptamer beacon fuel, and increased the detection efficiency and sensitivity. The signal was applied using the 2-aminopurine (2AP) molecule, the fluorescence of which could be quenched via stacking interaction with the adjacent bases in the beacon. Given its fluorescent properties, this design achieved low background analysis with high sensitivity. Chlorpyrifos was used as a model to explore the SFA proof-of-concept. By replacing the aptamer, this method can be extended to other pesticide molecules. Therefore, this study may become a powerful tool for quantitative detection of various targets for different purposes. •A self-fluorescence aptasensor based on Exo III-assisted CHA is presented for detection of chlorpyrifos.•Conformation change of aptamer-triggered signal amplification strategy proposed.•By using 2AP molecular, the aptasensor can achieve zero background analysis and further improve the sensitivity.•Applicable for sensing other non-nucleic acid targets, changing the aptamer in aptasensor.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2022.131441