Novel Simultaneous Removal of Ammonium and Sulfate by Isolated Bacillus cereus Strain from Sewage Treatment Plant

• Published in: Water, air, and soil pollution air, and soil pollution, 2022-06, Vol.233 (6), Article 185
• Main Authors: Mohammed Madani, Rayan, Liang, Jiyan, Cui, Li, Elsalahi, Randa H., Ayode Otitoju, Tunmise, Zhang, Dandan, Song, Xiaoxiong, Yongguang, Ma, Liu, Shiyue
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Ammonium; Ammonium compounds; Ammonium nitrogen; Ammonium sulfate; Anaerobic treatment; Atmospheric Protection/Air Quality Control/Air Pollution; Bacillus cereus; Bacteria; Biological products; Biomaterials; Biomedical materials; Climate Change/Climate Change Impacts; Earth and Environmental Science; Efficiency; Environment; Environmental monitoring; Hydrogeology; Industrial wastes; Industrial wastewater; Microbiological strains; Microorganisms; Nitrogen; Oxidation; pH effects; Phenols; Procedures; Purification; Removal; rRNA; Sewage; Sewage disposal; Sewage treatment; Sewage treatment plants; Soil Science & Conservation; Substrates; Sulfate reduction; Sulfates; Sulfur dioxide; Sulphate reduction; Temperature; Wastewater; Wastewater treatment; Wastewater treatment plants; Water Quality/Water Pollution; Water treatment; Water utilities
• ISSN: 0049-6979; 1573-2932
• DOI: 10.1007/s11270-022-05627-4

Sulfate-reducing anaerobic ammonium oxidation (SRAO) is one of the unique biochemical reactions involving the use of ammonium as the electron donor and sulfate as the electron acceptor. Bacillus cereus (named SUD-1 ) was isolated from industrial wastewater under an optimized anaerobic acclimation procedure for 14 days which is capable to be mixotrophic. The isolate was identified using the 16 S rRNA sequencing as Bacillus cereus FDAARGOS_798 with optimum growth pH and temperature of 7 and 30 ± 2 °C, respectively. The SUD-1 reached the maximum removal efficiency of 67% (NH + 4 ) and 80% (SO 2− 4 ) in a closed anaerobic batch for 10 days. Its optimum pH, temperature, isolate volume, and (NH 4 ) 2 SO 4 substrate concentration were 8, 30 °C, 5% (v/v), and 0.1 g/L, respectively. The result has shown the pronounced performance of SUD-1 strain as a novel biomaterial to simultaneously remove ammonium and sulfate which is an unconventional reaction and thus indicates a good potential for application in real wastewater treatment plants. Highlights Anaerobic acclimation procedure was used to obtain the Bacillus cereus , referred to as the SUD-1 strain. Successful sulfate-reducing anammox was achieved by time-reduced SUD -1 strain. Using the SUD-1 strain, the NH 4 + and SO 4 2− were simultaneously reduced by 67% and 80%, respectively. The SUD 1 strain was well-grown with improved degradation efficiency for high concentrations of NH 4 + and SO 4 2− . Graphical abstract