A novel 3D printing method for cell alignment and differentiation

The application of bioprinting allows precision deposition of biological materials for bioengineering applications. Here we propose a 2 stage methodology for bioprinting using a back pressure-driven, automated robotic dispensing system. This apparatus can prepare topographic guidance features for ce...

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Bibliographic Details
Published in:International journal of bioprinting 2024-08, Vol.1 (1), p.57
Main Authors: Bhuthalingam, Ramya, Lim, Pei Qi, A Irvine, Scott, Agrawal, Animesh, Mhaisalkar, Priyadarshini S, An, Jia, Chua, Chee Kai, Venkatraman, Subbu
Format: Article
Language:English
Subjects:
Automation
Bioengineering
Biological materials
CAD
Cell differentiation
Cell morphology
Computer aided design
Cytology
Deposition
Dispensing
Elongation
Etching
Fibroblasts
Flow cytometry
Gelatin
Grooves
Mesenchyme
Morphology
Orientation
Phenotypes
Polystyrene
Polystyrene resins
Robotics
Sine waves
Software
Stem cells
Styli
Three dimensional printing
Topography
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Summary:The application of bioprinting allows precision deposition of biological materials for bioengineering applications. Here we propose a 2 stage methodology for bioprinting using a back pressure-driven, automated robotic dispensing system. This apparatus can prepare topographic guidance features for cell orientation and then bioprint cells directly onto them. Topographic guidance features generate cues that influence adhered cell morphology and phenotype. The robotic dispensing system was modified to include a sharpened stylus that etched on a polystyrene surface. The same computer-aided design (CAD) software was used for both precision control of etching and bioink deposition. Various etched groove patterns such as linear, concentric circles, and sinusoidal wave patterns were possible. Fibroblasts and mesenchymal stem cells (MSC) were able to sense the grooves, as shown by their elongation and orientation in the direction of the features. The orientated MSCs displayed indications of lineage commitment as detected by fluorescence-activated cell sorting (FACS) analysis. A 2% gelatin bioink was then used to dispense cells onto the etched features using identical, programmed co-ordinates. The bioink allows the cells to contact sense the pattern while containing their deposition within the printed pattern.
ISSN:2424-7723
2424-8002
DOI:10.18063/IJB.2015.01.008