A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation

Background The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells. Nutrition is one of the key factors that either increases or decreases the risk of cancer. Mannose has been found in many fruits such as oranges, apples and berries. Mannose has been linked...

Full description

Saved in:
Bibliographic Details
Published in:Egyptian Journal of Medical Human Genetics 2020-11, Vol.21 (1), p.64-7
Main Authors: Mazlan, Muhammad Alif, Isa, Muhammad Lokman Md, Ibrahim, Moustafa
Format: Article
Language:English
Subjects:
Adenocarcinoma
Cancer
Cell viability
Colorectal cancer
Fibroblasts
Gene mutations
Genetic aspects
Health aspects
In vitro technique
Investigations
Malignant melanoma
Mannose
Melanoma
Monosaccharides
Oncology, Experimental
Point mutation
Risk factors
Skin cancer
Sugars
Tumor cell lines
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells. Nutrition is one of the key factors that either increases or decreases the risk of cancer. Mannose has been found in many fruits such as oranges, apples and berries. Mannose has been linked to increase the risk factors or potential therapeutic for cancers. However, insufficient information is available on the effects of high mannose concentration on the normal and cancer cell lines. This study aimed to evaluate the viability patterns of human cancer and normal cell lines treated with mannose. Human gingival fibroblast (HGF), skin malignant melanoma (A375) and colorectal adenocarcinoma (HT29) cell lines were cultured and treated with additional mannose in three respective concentrations: 1 mg/ml, 5 mg/ml and 10 mg/ml. Then, cell viability was measured using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-assay. Results The HGF cells' percentage pattern of viability showed a rapid decline of nearly 95% on the third day of treatment. A375 cells were able to survive in high mannose condition as the cell viability percentage was at the highest value on Day 5. Meanwhile, HT29 cells showed declining cell viability pattern when treated with mannose. The data exhibited significance; the p value was less than 0.001. Conclusions High mannose concentration can be toxic to HGF. In addition, A375 is adaptive to mannose at all concentrations in which it shares the same pattern with the untreated group. However, the cell viability pattern for HT29 cell is declining.
ISSN:1110-8630
2090-2441
DOI:10.1186/s43042-020-00109-w