Association between PON1 rs662 gene polymorphism and serum paraoxonase1 level in coronary artery disease patients in Northern India

• Published in: Egyptian Journal of Medical Human Genetics 2021-09, Vol.22 (1), p.74-8
• Main Authors: Kumar, Rohit, Saini, Vandana, Kaur, Charanjeet, Isser, H. S, Tyagi, Nitin, Sahoo, Subhra
• Format: Article
• Language: English
• Subjects: Analysis; Angiography; Atherogenesis; Cardiovascular disease; Coronary artery; Coronary artery disease; Coronary heart disease; Coronary vessels; Development and progression; Enzyme-linked immunosorbent assay; Enzymes; Ethylenediaminetetraacetic acid; Gene frequency; Gene polymorphism; Genes; Genetic aspects; Genetic polymorphisms; Genetic research; Health aspects; Heart diseases; High density lipoprotein; India; Lipid peroxidation; Medical imaging; Morbidity; Mortality; Oxidized LDL; Paraoxonase1; Polymorphism; Population; Population studies; rs662 gene polymorphism; Single-nucleotide polymorphism; Stenosis; Vein & artery diseases
• ISSN: 1110-8630; 2090-2441
• DOI: 10.1186/s43042-021-00196-3

Background Coronary artery disease (CAD) is a leading cause of morbidity and mortality, with a shifting trend towards the younger population. Paraoxonase1 (PON1) is a glycoprotein enzyme associated with high-density lipoprotein (HDL) particles in the blood. It has the ability to protect against lipid oxidation, thereby reducing the risk of atherogenesis. PON1 rs662 gene polymorphism may affect serum PON1 levels as well as its activity and may have a significant role in the pathogenesis of CAD. The present study was conducted to identify the association of PON1 rs662 gene polymorphism with serum PON1 levels in CAD patients in the North Indian population. This case-control study included 71 angiography-proven CAD patients (with > 50% luminal stenosis in one or more coronary arteries) and 71 controls (with < 50% luminal obstruction in angiography). PON1 rs662 gene polymorphism was studied using PCR and RFLP under the standardized protocol. Serum PON1 levels were estimated by ELISA. Results The serum PON1 level was significantly lower in the CAD group than in the controls (7.79 ± 3.16 vs. 10.79 ± 3.19 ng/mL; p < 0.0001). Logistic regression analysis showed that homozygous GG genotype of PON1 rs662 SNP has ninefold increased risk of developing CAD in an Indian population (OR = 9.0, 95%CI 2.79-29.06, p = 0.0002). A significantly higher frequency of G allele was also observed in CAD patients than in controls (OR 2.64, 95%CI 1.61-4.33, p = 0.001). Conclusions The reduced serum PON1 level is associated with CAD. PON1 rs662 gene polymorphism is significantly associated with CAD susceptibility in the North Indian population.