MicroRNA Profiling in Melanoma Cells That Are Resistant to Dacarbazine

One of the components of the tumor progression of melanoma of the skin is the formation of drug resistance. Chemotherapeutic agents are able to influence the cell cycle, DNA repair included, through the implementation of epigenetic mechanisms involving microRNAs, which are small noncoding RNA molecu...

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Bibliographic Details
Published in:Cell and tissue biology 2022, Vol.16 (3), p.203-212
Main Authors: Zinchenko, I. S., Palkina, N. V., Ruksha, T. G.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Carcinogenesis
Cell Biology
Cell cycle
Chemoresistance
Chemotherapy
Dacarbazine
DNA microarrays
DNA repair
Drug resistance
Epigenetics
Flow cytometry
Life Sciences
Melanoma
MicroRNAs
miRNA
Mitosis
Nucleotides
Signal transduction
Tumor cells
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Summary:One of the components of the tumor progression of melanoma of the skin is the formation of drug resistance. Chemotherapeutic agents are able to influence the cell cycle, DNA repair included, through the implementation of epigenetic mechanisms involving microRNAs, which are small noncoding RNA molecules 18–25 nucleotides long. They are able to regulate the onset and development of a tumor by changing the expression of genes of various signaling cascades. In this regard, the aim of our study was to determine the microRNA profile and the distribution of melanoma cells by cell-cycle phases when exposed to the chemotherapeutic agent dacarbazine, followed by bioinformatic analysis of the microRNA profile mediating the development of chemoresistance. To solve this problem, the BRO cell line was used, in which, after exposure to dacarbazine, the microRNA profile was assessed based on microarraying and the distribution of cells by cell-cycle phases was determined based on flow cytometry. The statistical significance of microRNA profile analysis data was calculated by analysis of variance, and the Benjamin–Hochberg multiple-comparison method was used to correct the significance levels of differences using the false-detection rate, the rest of the experiments were evaluated by the U criterion of Mann–Whitney. The results showed that, under the influence of dacarbazine, the proportion of viable tumor cells of the BRO line in the mitosis phase increases, with the expression of a number of microRNAs also changing. According to bioinformatic analysis, these microRNAs are involved in signal-transduction mechanisms that are exclusively related to carcinogenesis and are of key importance in the pathogenesis of melanoma.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X22030117