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Efficient label-free CTC enrichment using novel elevated height chip chamber by vortex technology
Circulating tumor cells (CTCs) are essential evidence for monitoring tumor dynamics and efficient cancer therapy. Besides its clinical importance, it is a feasible method for liquid biopsy in oncological studies. Vortex is a label-free technique using inertial microfluidic technology to separate CT...
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Published in: | Microfluidics and nanofluidics 2022-06, Vol.26 (6), Article 48 |
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description | Circulating tumor cells (CTCs) are essential evidence for monitoring tumor dynamics and efficient cancer therapy. Besides its clinical importance, it is a feasible method for liquid biopsy in oncological studies. Vortex is a label-free technique using inertial microfluidic technology to separate CTCs from blood with high throughput. A possible moderate separation efficiency arises due to the loss of particles from the vortex reservoir during particle-to-particle interactions. This study develops a novel three-dimensional reservoir geometry of the vortex with more efficient CTCs separation. In the proposed geometry, the height of the main channel is the same as the vortex chips and sets to 70 µm; however, the reservoir height increased to 100 µm. This design is called elevated height chip (EHC). The finite element simulation method is employed for optimizing reservoir height and working Reynolds number (Re). To verify the functionality of the EHC, the constant height chip design was also fabricated and showed a good correlation with the previously reported result. Both chips are tested with polystyrene beads (20, 15, and 8 µm) and MCF-7 cells. The results show 40% and 20% increase in efficiency for polystyrene beads and MCF-7 cells, respectively. Finally, it is demonstrated that 45% efficiency and 80% purity per cycle are achieved using the new proposed structure, and it is increased to 80% efficiency and 80% purity for over three cycles repetition. |
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Besides its clinical importance, it is a feasible method for liquid biopsy in oncological studies. Vortex is a label-free technique using inertial microfluidic technology to separate CTCs from blood with high throughput. A possible moderate separation efficiency arises due to the loss of particles from the vortex reservoir during particle-to-particle interactions. This study develops a novel three-dimensional reservoir geometry of the vortex with more efficient CTCs separation. In the proposed geometry, the height of the main channel is the same as the vortex chips and sets to 70 µm; however, the reservoir height increased to 100 µm. This design is called elevated height chip (EHC). The finite element simulation method is employed for optimizing reservoir height and working Reynolds number (Re). To verify the functionality of the EHC, the constant height chip design was also fabricated and showed a good correlation with the previously reported result. Both chips are tested with polystyrene beads (20, 15, and 8 µm) and MCF-7 cells. The results show 40% and 20% increase in efficiency for polystyrene beads and MCF-7 cells, respectively. Finally, it is demonstrated that 45% efficiency and 80% purity per cycle are achieved using the new proposed structure, and it is increased to 80% efficiency and 80% purity for over three cycles repetition.</description><identifier>ISSN: 1613-4982</identifier><identifier>EISSN: 1613-4990</identifier><identifier>DOI: 10.1007/s10404-022-02553-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Beads ; Biomedical Engineering and Bioengineering ; Biopsy ; Design ; Efficiency ; Engineering ; Engineering Fluid Dynamics ; Finite element method ; Fluid flow ; Height ; Mathematical models ; Microfluidics ; Nanotechnology and Microengineering ; Neoplasms ; Particle interactions ; Polystyrene ; Polystyrene resins ; Purity ; Research Paper ; Reynolds number ; Separation ; Technology ; Tumor cells ; Tumors ; Vortices</subject><ispartof>Microfluidics and nanofluidics, 2022-06, Vol.26 (6), Article 48</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c234z-38b9a6625e7148b20356b893909a6c019c55241f27afa353a0771eb4c04727223</citedby><cites>FETCH-LOGICAL-c234z-38b9a6625e7148b20356b893909a6c019c55241f27afa353a0771eb4c04727223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Amini, A.</creatorcontrib><creatorcontrib>Hajghassem, H.</creatorcontrib><creatorcontrib>Nikfarjam, A.</creatorcontrib><creatorcontrib>Yarahmadi, N.</creatorcontrib><creatorcontrib>Mohamadsharifi, A.</creatorcontrib><creatorcontrib>HajiMohammadHoseyni, F.</creatorcontrib><creatorcontrib>Moradi, N.</creatorcontrib><title>Efficient label-free CTC enrichment using novel elevated height chip chamber by vortex technology</title><title>Microfluidics and nanofluidics</title><addtitle>Microfluid Nanofluid</addtitle><description>Circulating tumor cells (CTCs) are essential evidence for monitoring tumor dynamics and efficient cancer therapy. Besides its clinical importance, it is a feasible method for liquid biopsy in oncological studies. Vortex is a label-free technique using inertial microfluidic technology to separate CTCs from blood with high throughput. A possible moderate separation efficiency arises due to the loss of particles from the vortex reservoir during particle-to-particle interactions. This study develops a novel three-dimensional reservoir geometry of the vortex with more efficient CTCs separation. In the proposed geometry, the height of the main channel is the same as the vortex chips and sets to 70 µm; however, the reservoir height increased to 100 µm. This design is called elevated height chip (EHC). The finite element simulation method is employed for optimizing reservoir height and working Reynolds number (Re). To verify the functionality of the EHC, the constant height chip design was also fabricated and showed a good correlation with the previously reported result. Both chips are tested with polystyrene beads (20, 15, and 8 µm) and MCF-7 cells. The results show 40% and 20% increase in efficiency for polystyrene beads and MCF-7 cells, respectively. Finally, it is demonstrated that 45% efficiency and 80% purity per cycle are achieved using the new proposed structure, and it is increased to 80% efficiency and 80% purity for over three cycles repetition.</description><subject>Analytical Chemistry</subject><subject>Beads</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biopsy</subject><subject>Design</subject><subject>Efficiency</subject><subject>Engineering</subject><subject>Engineering Fluid Dynamics</subject><subject>Finite element method</subject><subject>Fluid flow</subject><subject>Height</subject><subject>Mathematical models</subject><subject>Microfluidics</subject><subject>Nanotechnology and Microengineering</subject><subject>Neoplasms</subject><subject>Particle interactions</subject><subject>Polystyrene</subject><subject>Polystyrene resins</subject><subject>Purity</subject><subject>Research Paper</subject><subject>Reynolds number</subject><subject>Separation</subject><subject>Technology</subject><subject>Tumor cells</subject><subject>Tumors</subject><subject>Vortices</subject><issn>1613-4982</issn><issn>1613-4990</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9UMlOwzAQtRBIlMIPcLLEOTDeshxRVBYJiUs5W46ZLFXqFDutaL8elyC4cZhFM--90TxCrhncMoDsLjCQIBPgPIZSIjmckBlLmUhkUcDpb5_zc3IRwgpAZpzBjJhFXXe2QzfS3lTYJ7VHpOWypOh8Z9v1cbMNnWuoG3bYU-xxZ0Z8py12TTtS23abmMy6Qk-rPd0NfsRPOqJt3dAPzf6SnNWmD3j1U-fk7WGxLJ-Sl9fH5_L-JbFcyEMi8qowacoVZkzmFQeh0iovRAFxbIEVVikuWc0zUxuhhIEsY1hJe_wk41zMyc2ku_HDxxbDqFfD1rt4UvM0aipIBYsoPqGsH0LwWOuN79bG7zUDfbRST1bqaKX-tlIfIklMpBDBrkH_J_0P6wtS3nZC</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Amini, A.</creator><creator>Hajghassem, H.</creator><creator>Nikfarjam, A.</creator><creator>Yarahmadi, N.</creator><creator>Mohamadsharifi, A.</creator><creator>HajiMohammadHoseyni, F.</creator><creator>Moradi, N.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TB</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H96</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>L6V</scope><scope>M0S</scope><scope>M7S</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>S0W</scope></search><sort><creationdate>20220601</creationdate><title>Efficient label-free CTC enrichment using novel elevated height chip chamber by vortex technology</title><author>Amini, A. ; 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Besides its clinical importance, it is a feasible method for liquid biopsy in oncological studies. Vortex is a label-free technique using inertial microfluidic technology to separate CTCs from blood with high throughput. A possible moderate separation efficiency arises due to the loss of particles from the vortex reservoir during particle-to-particle interactions. This study develops a novel three-dimensional reservoir geometry of the vortex with more efficient CTCs separation. In the proposed geometry, the height of the main channel is the same as the vortex chips and sets to 70 µm; however, the reservoir height increased to 100 µm. This design is called elevated height chip (EHC). The finite element simulation method is employed for optimizing reservoir height and working Reynolds number (Re). To verify the functionality of the EHC, the constant height chip design was also fabricated and showed a good correlation with the previously reported result. Both chips are tested with polystyrene beads (20, 15, and 8 µm) and MCF-7 cells. The results show 40% and 20% increase in efficiency for polystyrene beads and MCF-7 cells, respectively. Finally, it is demonstrated that 45% efficiency and 80% purity per cycle are achieved using the new proposed structure, and it is increased to 80% efficiency and 80% purity for over three cycles repetition.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s10404-022-02553-z</doi></addata></record> |
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subjects | Analytical Chemistry Beads Biomedical Engineering and Bioengineering Biopsy Design Efficiency Engineering Engineering Fluid Dynamics Finite element method Fluid flow Height Mathematical models Microfluidics Nanotechnology and Microengineering Neoplasms Particle interactions Polystyrene Polystyrene resins Purity Research Paper Reynolds number Separation Technology Tumor cells Tumors Vortices |
title | Efficient label-free CTC enrichment using novel elevated height chip chamber by vortex technology |
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