Simple and rapid method for molecular identification of Konosirus punctatus and Clupanodon thrissa

Konosirus punctatus and Clupanodon thrissa are distributed in overlapping geographical areas. Their similar appearance makes it difficult to distinguish them by morphology. In recent years, overfishing has led to frequent stock collapses. The development of resource management and identification tec...

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Bibliographic Details
Published in:Conservation genetics resources 2022-06, Vol.14 (2), p.163-165
Main Authors: Li, Wei, Lei, Luo, Zhu, Junxian, Chen, Haigang, Chen, Chen, Wang, Yakun, Geng, Lulu, Chen, Fangcan, Zhu, Xinping
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Binding sites
Biodiversity
Biomedical and Life Sciences
Clupanodon thrissa
Conservation Biology/Ecology
Cytochrome
Ecology
Evolutionary Biology
Fisheries
Genetic resources
Genetics
Genomes
Identification
Konosirus punctatus
Life Sciences
Mitochondrial DNA
Morphology
Phylogenetics
Plant Genetics and Genomics
Resource management
Species
Technical Note
Wildlife conservation
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Summary:Konosirus punctatus and Clupanodon thrissa are distributed in overlapping geographical areas. Their similar appearance makes it difficult to distinguish them by morphology. In recent years, overfishing has led to frequent stock collapses. The development of resource management and identification techniques is necessary for accurate statistical assessment of harvests and to protect the diversity of species and genetic resources. Considering the difficulty of distinguishing these two species, a molecular marker technique was developed to identify them. As a result, primer HB3 was developed to obtain amplification products for C. thrissa , which are not produced for K. punctatus . This study allows rapid distinction between these two species based on amplification products, providing a simple and rapid molecular method for checking products sold and illegally caught.
ISSN:1877-7260
1877-7252
1877-7260
DOI:10.1007/s12686-022-01267-2