Production of Diadinoxanthin in an Intensive Culture of the Diatomaceous Alga Cylindrotheca closterium (Ehrenb.) Reimann et Lewin. and Its Proapoptotic Activity

The production of diadinoxanthin and its cytostatic activity in an intensive culture of C. closterium has been estimated via molecular modeling and experiments with the human tumor cell cultures OVCAR5, OVCAR8, KURAMOCHI, and OVSAHO. According to data from both the simulation and experiments with th...

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Published in:Applied biochemistry and microbiology 2022-06, Vol.58 (3), p.261-268
Main Authors: Gevorgiz, R. G., Gureev, M. A., Zheleznova, S. N., Gureeva, E. V., Nechoroshev, M. V.
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Biomedical and Life Sciences
Cell culture
Cylindrotheca closterium
Diatomaceous earth
Fucoxanthin
Life Sciences
Medical Microbiology
Microbiology
Molecular modelling
Ovarian cancer
Tumors
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Summary:The production of diadinoxanthin and its cytostatic activity in an intensive culture of C. closterium has been estimated via molecular modeling and experiments with the human tumor cell cultures OVCAR5, OVCAR8, KURAMOCHI, and OVSAHO. According to data from both the simulation and experiments with the cell cultures, diadinoxanthin demonstrates lower activity (by more than four times) in comparison with fucoxanthin. The IC 50 value for diadinoxanthin is achieved at a concentration of more than 100 μM, while fucoxanthin exhibits a cytostatic effect below 18.75 μM. In a flow-through culture of C. closterium , the production of diadinoxanthin can exceed the production of fucoxanthin by 14 times, which can make the technology for its production less expensive and can significantly reduce the cost of therapeutic and prophylactic drugs based on microalgae.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683822010033