Thromboresistance of Polyurethane Plates Modified with Quaternized Chitosan and Heparin

To obtain modified polyurethane (PU) plates, quaternized chitosan (QuatCh; degree of substitution 50%, degree of deacetylation 87%) and unfractionated heparin (UFH) were applied in layers to the plate surfaces. The relief of the surface of the plates was studied via atomic-force microscopy (AFM). Af...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and microbiology 2022-06, Vol.58 (3), p.315-321
Main Authors: Drozd, N. N., Lunkov, A. P., Shagdarova, B. Ts, Zhuikova, Yu. V., Il’ina, A. V., Varlamov, V. P.
Format: Article
Language:English
Subjects:
Anticoagulants
Atomic force microscopy
Biochemistry
Biomedical and Life Sciences
Blood
Blood clots
Blood coagulation
Chitosan
Contact angle
Deacetylation
Heparin
High resistance
Incubation
Life Sciences
Medical Microbiology
Microbiology
Plates
Polyurethane
Polyurethane resins
Unevenness
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To obtain modified polyurethane (PU) plates, quaternized chitosan (QuatCh; degree of substitution 50%, degree of deacetylation 87%) and unfractionated heparin (UFH) were applied in layers to the plate surfaces. The relief of the surface of the plates was studied via atomic-force microscopy (AFM). After processing, the root-mean-square roughness and the height of the unevenness of the PU profile of the plates significantly increased. The value of the contact angle of the modified PU plates decreased from 86.1° ± 11.3° to 63.2° ± 3.68°. Donor blood and the blood recalcification time test were used to assess the in vitro resistance of the PU plates to the appearance of blood clots on the surface (thromboresistance, TR). When the modified PU plates were incubated with blood for 20–180 min, the TR reached 78.43 ± 4.02–88.94 ± 1.98%. The recalcification time of human blood containing PU plates coated with QuatCh–UFH (483 ± 22.64 s for 20 min of incubation and576.5 ± 24.64 s for 40 min of incubation) significantly differed from the recalcification time of human blood containing PU plates without coating (257.5 ± 12.84 s for 20 min of incubation and 273.3 ± 16.6 s for 40 min of incubation). Thus, layer-by-layer modification of the PU plates with QuatCh and UFH led to a high resistance to the appearance of blood clots on the surface.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683822020041