Production of tetraploid and octoploid Haemanthus albiflos plants using immature embryo-derived embryogenic calli treated with colchicine

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2022-06, Vol.149 (3), p.747-752
• Main Authors: Nakano, Arisa, Hoshino, Yoichiro
• Format: Article
• Language: English
• Subjects: Benzyladenine; Biomedical and Life Sciences; Biotechnology of Ornamental Plants; Callus; Colchicine; Diploids; Embryonic growth stage; Embryos; Flowers & plants; Gellan gum; Growth regulators; Leaves; Life Sciences; Picloram; Plant Genetics and Genomics; Plant growth; Plant Pathology; Plant Physiology; Plant Sciences; Protocol Paper; Somatic embryogenesis; Sucrose
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-022-02267-8

A protocol was developed to produce tetraploid and octoploid Haemanthus albiflos Jacq. (Amaryllidaceae) plants by treating with colchicine to diploid immature embryo-derived embryogenic calli. The calli were induced from immature embryos cultured on 3 g L −1 gellan gum-solidified Murashige and Skoog (MS) medium containing 5 mg L ‐1 each of picloram and 6-benzylaminopurine, and 30 g L −1 sucrose. Treatment with 0.2% (w/v) colchicine to the calli was performed with different durations of 24, 48, and 72 h. Tetraploids and octoploid plants were induced through somatic embryogenesis on a half-strength MS medium without plant growth regulators. Key message In diploid Haemanthus albiflos , immature embryo-derived embryogenic callus cultures were established. After colchicine treatment to the embryogenic callus cultures, tetraploid and octoploid plants were obtained simultaneously. Graphical abstract