Production of tetraploid and octoploid Haemanthus albiflos plants using immature embryo-derived embryogenic calli treated with colchicine

A protocol was developed to produce tetraploid and octoploid Haemanthus albiflos Jacq. (Amaryllidaceae) plants by treating with colchicine to diploid immature embryo-derived embryogenic calli. The calli were induced from immature embryos cultured on 3 g L −1 gellan gum-solidified Murashige and Skoog...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2022-06, Vol.149 (3), p.747-752
Main Authors: Nakano, Arisa, Hoshino, Yoichiro
Format: Article
Language:English
Subjects:
Benzyladenine
Biomedical and Life Sciences
Biotechnology of Ornamental Plants
Callus
Colchicine
Diploids
Embryonic growth stage
Embryos
Flowers & plants
Gellan gum
Growth regulators
Leaves
Life Sciences
Picloram
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Physiology
Plant Sciences
Protocol Paper
Somatic embryogenesis
Sucrose
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Hoshino, Yoichiro
description A protocol was developed to produce tetraploid and octoploid Haemanthus albiflos Jacq. (Amaryllidaceae) plants by treating with colchicine to diploid immature embryo-derived embryogenic calli. The calli were induced from immature embryos cultured on 3 g L −1 gellan gum-solidified Murashige and Skoog (MS) medium containing 5 mg L ‐1 each of picloram and 6-benzylaminopurine, and 30 g L −1 sucrose. Treatment with 0.2% (w/v) colchicine to the calli was performed with different durations of 24, 48, and 72 h. Tetraploids and octoploid plants were induced through somatic embryogenesis on a half-strength MS medium without plant growth regulators. Key message In diploid Haemanthus albiflos , immature embryo-derived embryogenic callus cultures were established. After colchicine treatment to the embryogenic callus cultures, tetraploid and octoploid plants were obtained simultaneously. Graphical abstract
doi_str_mv 10.1007/s11240-022-02267-8
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ispartof Plant cell, tissue and organ culture, 2022-06, Vol.149 (3), p.747-752
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1573-5044
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source Springer Nature
subjects Benzyladenine
Biomedical and Life Sciences
Biotechnology of Ornamental Plants
Callus
Colchicine
Diploids
Embryonic growth stage
Embryos
Flowers & plants
Gellan gum
Growth regulators
Leaves
Life Sciences
Picloram
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Physiology
Plant Sciences
Protocol Paper
Somatic embryogenesis
Sucrose
title Production of tetraploid and octoploid Haemanthus albiflos plants using immature embryo-derived embryogenic calli treated with colchicine
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