Characterization of digestive enzymes from sandfish, Holothuria scabra juvenile

Understanding the specific role of enzyme activity, such as digestive enzymes present in the intestine of Holothuria scabra juvenile, combined with an understanding of the animal habits and their digestive capacity is essential in the development of an appropriate diet. Juvenile sandfish with an ave...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2022-06, Vol.1033 (1), p.12016
Main Authors: Sembiring, S B M, Hutapea, J H, Giri, I N A, Haryanti, Pratiwi, R, Hadisusanto, S
Format: Article
Language:English
Subjects:
Amylases
Digestive enzymes
Enzymatic activity
Enzyme activity
Enzymes
Holothuria scabra
Intestine
Lipase
pH effects
Protease
Proteinase
Sandfish
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Summary:Understanding the specific role of enzyme activity, such as digestive enzymes present in the intestine of Holothuria scabra juvenile, combined with an understanding of the animal habits and their digestive capacity is essential in the development of an appropriate diet. Juvenile sandfish with an average weight and total length of 2.81 ± 0.54 g and 4.10 ± 0.54 cm were collected and its intestine was used to analyze the activity of protease, amylase, and lipase enzymes. Parameters testing for all enzyme activities were conducted at 12 levels of pH (1.0; 2.0; 3.0; 4.0; 5.0; 6.0; 7.0; 8.0; 9.0; 10.0; 11.0; 12.0) and temperature 8 levels (10; 20; 30; 40; 50; 60; 70; 80; 90°C). Optimum pH for protease, amylase, and lipase activity were 7.0, 8.0, and 3.0, respectively, while optimum temperatures were 50, 50, and 40°C, respectively. This information about optimum pH and temperature in the biology of sandfish is useful as a basis for further studies on feeding physiology.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/1033/1/012016