Dispersion and degradation of environmental DNA from caged fish in a marine environment

Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, t...

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Bibliographic Details
Published in:Fisheries science 2019-03, Vol.85 (2), p.327-337
Main Authors: Murakami, Hiroaki, Yoon, Seokjin, Kasai, Akihide, Minamoto, Toshifumi, Yamamoto, Satoshi, Sakata, Masayuki K., Horiuchi, Tomoya, Sawada, Hideki, Kondoh, Michio, Yamashita, Yoh, Masuda, Reiji
Format: Article
Language:English
Subjects:
Biodegradation
Biomass
Biomedical and Life Sciences
Cages
Censuses
Coastal environments
Deoxyribonucleic acid
Dispersion
DNA
Environmental degradation
Environmental DNA
Fish
Fish & Wildlife Biology & Management
Fisheries
Food Science
Freshwater & Marine Ecology
Life Sciences
Marine environment
Marine fish
Marine fishes
Nucleotide sequence
Original Article
PCR
Research centers
Science education
Sea surface
Surface water
Temperature (air-sea)
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Summary:Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-018-1282-6