Optical diffraction tomography meets metrology — Measurement accuracy on cellular and subcellular level

Three-dimensional quantitative phase imaging (3D QPI) delivers volumetric information about the refractive index distribution within microobjects. Such imaging and measurement capabilities are of great interest for technical and biomedical applications, yet there are no standardized methods for test...

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Bibliographic Details
Published in:Measurement : journal of the International Measurement Confederation 2022-05, Vol.195, p.111106, Article 111106
Main Authors: Ziemczonok, Michał, Kuś, Arkadiusz, Kujawińska, Małgorzata
Format: Article
Language:English
Subjects:
3-D technology
Biomedical materials
Diffraction
Direct laser writing
Metrology
Optical diffraction tomography
Phantom
Physical properties
Quality assessment
Quantitative phase imaging
Refractive index
Refractivity
Standardization
Tomography
Volumetric analysis
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Summary:Three-dimensional quantitative phase imaging (3D QPI) delivers volumetric information about the refractive index distribution within microobjects. Such imaging and measurement capabilities are of great interest for technical and biomedical applications, yet there are no standardized methods for testing and reporting metrological performance. Proper metrology is crucial for further developments in QPI and for cross-referencing a range of physical properties with other laboratories and techniques. In this paper we present methodology for metrological evaluation of the 3D QPI instruments for single-cell and subcellular structures. The methodology entails suitable phantom, its digital twin, quality assessment metric and easily reproducible protocol that is attainable for both numerical and experimental analysis. We demonstrate its applicability by comparing simulated reconstructions with measurement results from 3 optical diffraction tomography systems. The results will serve as a reference point for past and future research, encourage to benchmark the new systems in a similar manner and further the efforts towards the standardization in 3D QPI metrology. [Display omitted] •Methodology for quality assessment of 3D refractive index reconstruction of cells.•Quantified impact of key system parameters on the reconstruction quality.•End-to-end evaluation enabled by the physical phantom and its digital twin.
ISSN:0263-2241
1873-412X
DOI:10.1016/j.measurement.2022.111106