A mechanistic study on electro-Fenton system cooperating with phangerochate chrysosporium to degrade lignin

• Published in: RSC advances 2022-06, Vol.12 (27), p.17285-17293
• Main Authors: Qin, Yingjian, Wang, Na, Ma, Zhongmin, Li, Jinsheng, Wang, Yaozong, Zang, Lihua
• Format: Article
• Language: English
• Subjects: Catalysis; Chemistry; Degradation; Functional groups; Industrial applications; Lignin; Manganese; NMR; Nuclear magnetic resonance; Peroxidase; White rot fungi
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d2ra01763f

The combined catalytic system of Electro-Fenton (E-Fenton) and Phanerochaete chrysosporium ( P. chrysosporium ) was constructed in liquid medium with additional potential to overcome the limitations of lignin degradation by white rot fungi alone. To further understand the mechanism of synergistic catalysis, we optimized the optimum potential for lignin catalysis by P. chrysosporium and built synergistic versus separate catalyses. After 48 h of incubation, the optimum growth environment and the highest lignin degradation rate (43.8%) of P. chrysosporium were achieved when 4 V was applied. After 96 h, the lignin degradation rate of the cocatalytic system was 62% (E-Fenton catalysis alone 22% and P. chrysosporium catalysis alone 19%), the pH of the growth maintenance system of P. chrysosporium was approximately 3.5, and the lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) enzyme activities, were significantly better than those of the control. The qPCR results indicated that the expression of both MnP and LiP genes was higher in the cocatalytic system. Meanwhile, FTIR and 2D-HSQC NMR confirmed that the synergistic catalysis was effective in breaking the aromatic functional groups and the side chains of the aliphatic region of lignin. This study showed that the synergistic catalytic process of electro-Fenton and P. chrysosporium was highly efficient in the degradation of lignin. In addition, the synergetic system is simple to operate, economical and green, and has good prospects for industrial application. The combined catalytic system of Electro-Fenton (E-Fenton) and Phanerochaete chrysosporium ( P. chrysosporium ) was constructed in liquid medium with additional potential to overcome the limitations of lignin degradation by white rot fungi alone.