Effectiveness of antagonistic bacteria, commercial fungicides, and fourth generation quaternary ammonium salts, against Fusarium oxysporum f. sp. cubense race “1 or 2”

• Published in: European journal of plant pathology 2022-07, Vol.163 (3), p.719-731
• Main Authors: Florencio-Anastasio, José Guadalupe, García-Ávila, Clemente de Jesús, Alarcón, Alejandro, Ferrera-Cerrato, Ronald, Quezada-Salinas, Andres, Almaraz-Suárez, Juan J., Moreno-Velázquez, Magnolia, Hernández-Ramos, Lervin
• Format: Article
• Language: English
• Subjects: Agriculture; Ammonium; Ammonium salts; Bacteria; Benomyl; Biomedical and Life Sciences; Carbendazim; Cultivars; Ecology; Fruits; Fungi; Fungicides; Fusarium oxysporum; Inoculation; Life Sciences; Mycelia; Paenibacillus; Pesticides; Plant Pathology; Plant Sciences; Plantations; Quaternary ammonium salts; Salts; Serratia; Strains (organisms); Tebuconazole
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-022-02510-0

There are three races of Fusarium oxysporum f.sp. cubense (Foc) that significantly affect commercial banana plantations. Most banana cultivars are very susceptible to infection by tropical race 4 of Foc (FocR4T); in contrast, some banana varieties (Cavendish group) are resistant to FocR1 and FocR2. Therefore, it is necessary to develop combined management strategies for controlling the imminent arrival of FocR4T in Mexican plantations. This research evaluated, under in vitro conditions, the effect of seven antagonist bacterial strains, six systemic fungicides, and a fourth generation quaternary ammonium salt on the mycelial development of five Foc strains isolated from banana plantations at Puebla, Michoacan and Oaxaca states (Mexico). Paenibacillus sp. (BSP 1.1) resulted in the highest antagonistic effect (46.6% inhibition) towards the five Foc strains, followed by Serratia sp. (AC35) with 26.6% fungal inhibition. Tebuconazole applied in doses of 50, 100 and 500 μg mL −1 of active ingredient inhibited fungal growth by 100%, whereas Carbendazim and Benomyl delivered 99% fungal inhibition. A high dose of fourth generation quaternary ammonium salts (1250 μg mL −1 ) caused fungal growth inhibition of 100% and 98.5% after 5 and 8 days post inoculation, respectively. Overall, Paenibacillus sp. (BSP 1.1) and Serratia sp. (AC35) were the most effective bacterial strains; moreover, the application of certain doses of systemic fungicides such as Tebuconazol (50 μg mL −1 ), Carbendazim and Benomyl (>500 μg mL −1 ), and ammonium quaternary salts at doses of 1250 μg mL −1 may be considered as alternatives for preventing dissemination, or for using them as part of FocR4T control and sanitary programs.