Biofilm Structural and Functional Features on Microplastic Surfaces in Greenhouse Agricultural Soil

• Published in: Sustainability 2022-06, Vol.14 (12), p.7024
• Main Authors: Chen, Yue, Wang, Xiaobing, Wang, Xiaoli, Cheng, Tong, Fu, Kuankuan, Qin, Zhentian, Feng, Ke
• Format: Article
• Language: English
• Subjects: Agricultural land; Agricultural production; Agriculture; Bacteria; Biodegradation; Biofilms; Chemoheterotrophy; Contaminants; Eps protein; Extracellular polymers; Farms; Fertilizers; Greenhouses; High density polyethylenes; Low density polyethylenes; Microorganisms; Microplastics; Nutrient cycles; Nutrients; Organic contaminants; Organic fertilizers; Organic materials; Organic wastes; Polyethylene; Polyethylene terephthalate; Polymers; Polysaccharides; Polystyrene resins; Presenilin 1; Relative abundance; Sludge; Soil contamination; Soil microorganisms; Soil nutrients; Structure-function relationships; Sustainability
• ISSN: 2071-1050; 2071-1050
• DOI: 10.3390/su14127024

Microplastics (MPs) enter the soil through a variety of pathways, including plastic mulching, sludge, and organic manure application. In recent years, domestic and foreign experts and scholars have been concerned about the residues and contamination of MPs in the soil of greenhouse agriculture. In this investigation, five types of MPs including low-density polyethylene (LDPE), high-density polyethylene (HDPE), polystyrene (PS), polypropylene (PP), and polyethylene terephthalate (PET), and two concentrations (1% and 5%, w/w) were used in a 30-day external exposure test. Evidence of microbial enrichment was found on the surface of the MPs. The total amount of biofilm on the surface of MPs increased dramatically with increasing exposure time and MP concentrations. The polysaccharide content of extracellular polymers (EPS) in biofilms was significantly different, and the maximum PS1 (1% (w/w) PS) concentration was 50.17 mg/L. However, EPS protein content did not change significantly. The dominant bacteria on the surface of MPs with different types and concentrations were specific, and the relative abundance of Patescibacteria was significantly changed at the phylum level. At the genus level, Methylophaga, Saccharimonadales, and Sphingomonas dominated the flora of LDPE1 (1% (w/w) LDPE), PS1, and PET5 (5% (w/w) PET). The dominant bacteria decompose organic materials and biodegrade organic contaminants. According to the FAPROTAX functional prediction study, chemoheterotrophy and aerobic chemoheterotrophyplay a role in ecosystem processes such as carbon cycle and climate regulation. The application of LDPE1 has a greater impact on the carbon cycle. Plant development and soil nutrients in greenhouse agriculture may be influenced by the interaction between MPs and microorganisms in the growing area, while MP biofilms have an impact on the surrounding environment and pose an ecological hazard.