Chelidonium majus crude extract induces activation of peripheral blood mononuclear cells and enhances their cytotoxic effect toward HeLa cells

The aim of the study was to examine the immunomodulatory effect of crude Chelidonium majus L ethanolic extract on ex vivo harvested peripheral blood mononuclear cells (PBMNCs). PBMNCs were isolated by density gradient centrifugation. The PBMNC cytotoxicity assay was performed with HeLa tumor cells a...

Full description

Saved in:
Bibliographic Details
Published in:International journal of environmental health research 2022-07, Vol.32 (7), p.1554-1566
Main Authors: Popovic, Ana, Deljanin, Milena, Popovic, Suzana, Todorovic, Danijela, Djurdjevic, Predrag, Matic, Sanja, Stankovic, Milan, Avramovic, Dusko, Baskic, Dejan
Format: Article
Language:English
Subjects:
Blood
CD14 antigen
CD4 antigen
CD57 antigen
CD8 antigen
Cell activation
Cell proliferation
Centrifugation
Chelidonium majus
Chelidonium majus L ethanolic extract
Cytotoxicity
cytotoxicity assay
Flow cytometry
Immunomodulation
Immunostimulation
Leukocytes (mononuclear)
Lymphocytes
Lymphocytes B
Lymphocytes T
Monocytes
PBMNC
Peripheral blood mononuclear cells
Perturbation
Subpopulations
Toxicity
Tumor cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the study was to examine the immunomodulatory effect of crude Chelidonium majus L ethanolic extract on ex vivo harvested peripheral blood mononuclear cells (PBMNCs). PBMNCs were isolated by density gradient centrifugation. The PBMNC cytotoxicity assay was performed with HeLa tumor cells as target cells. MTT assay was used to estimate the proliferation effect of extract and cytotoxic efficiency of treated PBMNCs. Flow cytometric analysis was used for immunophenotyping. Treatment induced moderate proliferative response, perturbation in PBMNC ratios, and the emergence of some unconventional subpopulations. The percentage ratio of double positive CD4+ and CD8+ T lymphocytes and monocytes, ratio of T and B lymphocytes expressing CD14, and percentage of NK cells expressing CD57 increased after treatment, indicating activation of PBMNC subpopulations. Cytotoxic activity against HeLa cells was enhanced. Activation of PBMNCs and enhancement of their cytotoxic effect toward HeLa cells indicate the immunostimulatory effect of Ch. majus ethanolic extract.
ISSN:0960-3123
1369-1619
DOI:10.1080/09603123.2021.1897534