First report of Passion fruit green spot virus infecting hibiscus plants

Ultrathin sections from lesions were examined in a JEM 1011 (JEOL, Japan) transmission electron microscope. Transmission electron micrographs of ultrathin sections of symptomatic leaf samples of hibiscus plants collected in the State of São Paulo, Brazil, samples Prb02 (A), Jbl01 (B), and SBO01 (C)....

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Bibliographic Details
Published in:New disease reports 2022-04, Vol.45 (2), p.n/a
Main Authors: da Costa‐Rodrigues, M., Rossetto Pereira, L., Ramos‐González, P. L., Chabi‐Jesus, C., Tassi, A. D., Leão, I. G., Harakava, R., Kitajima, E. W., Freitas‐Astúa, J.
Format: Article
Language:English
Subjects:
Brevipalpus‐transmitted viruses
Cilevirus
Complementary DNA
Dichorhavirus
Endoplasmic reticulum
Kitaviridae
Maximum likelihood method
Micrography
Phylogenetics
RNA viruses
RNA-directed DNA polymerase
Viruses
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Summary:Ultrathin sections from lesions were examined in a JEM 1011 (JEOL, Japan) transmission electron microscope. Transmission electron micrographs of ultrathin sections of symptomatic leaf samples of hibiscus plants collected in the State of São Paulo, Brazil, samples Prb02 (A), Jbl01 (B), and SBO01 (C). Arrows indicate virus particles; N: nucleus RNA extracts were prepared from individual lesions (c. 100 mg) using TRIzol™ reagent (Life Technologies, USA) from which cDNA solutions were prepared using a GoScript™ Reverse Transcriptase kit (Promega, USA) and a mix of random primers.
ISSN:2044-0588
2044-0588
DOI:10.1002/ndr2.12080