CpG Island Methylation of the Rap1Gap Gene in Medullary Thyroid Cancer

Background: Medullary thyroid cancer (MTC) is a rare type of neuroendocrine tumor. This study aimed to investigate the gene and protein expression of RAP1GAP and DNA methylation patterns of its CpG74a , CpG74b , and CpG24 in an Iranian population with MTC. Methods: In this case-control study, we sel...

Full description

Saved in:
Bibliographic Details
Published in:Archives of Iranian medicine 2022-03, Vol.25 (3), p.171-177
Main Authors: Faam, Bita, Ghadiri, Ata. A, Ghaffari, Mohammad Ali, Totonchi, Mehdi, Amouzegar, Atieh, Azizi, Fereidoun, Shahbazian, Hajiehbibi, Hashemitabar, Mahmoud, Fanaei, Seyed Ahmad, Khorsandi, Layasadat
Format: Article
Language:English
Subjects:
DNA methylation
Epidemiology
Gene expression
Proteins
Thyroid cancer
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Medullary thyroid cancer (MTC) is a rare type of neuroendocrine tumor. This study aimed to investigate the gene and protein expression of RAP1GAP and DNA methylation patterns of its CpG74a , CpG74b , and CpG24 in an Iranian population with MTC. Methods: In this case-control study, we selected 55 individuals who underwent thyroidectomy in Erfan hospital, Tehran, between 2018 and 2020. Samples were divided into normal thyroid tissues (control; n=20), benign nodule (n=20), and MTC (n=15). DNA methylation patterns were investigated using MSP (methylation-specific PCR). The protein level and mRNA expression of RAP1GAP were also evaluated using western blotting and real-time PCR, respectively. Results: The hyper-methylation rates of CpG24 and CpG74a in the MTC samples were considerably higher than the controls (83% versus 15% and 74% versus 17%, respectively; P
ISSN:1029-2977
1735-3947
DOI:10.34172/aim.2022.29