Impact of Static Magnetic Field on the Callogenesis, Phytochemical Production and Antioxidant enzymes in Anthemis gilanica

In the present study, impact of static magnetic field (SMF) on callus growth, lipid peroxidation and antioxidative defense system of Anthemis gilanica were investigated. The in vitro hypocotyl explants were placed in Murashige and Skoog medium supplemented with 0.5 mg L –1 of kinetin and 2 mg L –1 o...

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Bibliographic Details
Published in:Russian journal of plant physiology 2022-08, Vol.69 (4), Article 77
Main Authors: Nasiri, M., Hassanpour, H., Sorahinobar, M., Niknam, V.
Format: Article
Language:English
Subjects:
Anthemis
Anthocyanins
Antioxidants
Ascorbic acid
Bioactive compounds
Biomedical and Life Sciences
Callus
Catalase
Enzymatic activity
Explants
Flavonoids
Flavonols
Free radicals
Herbal medicine
Hydrogen peroxide
Isoenzymes
Isoforms
Kinetin
L-Ascorbate peroxidase
Life Sciences
Lipid peroxidation
Lipids
Magnetic fields
Medicinal plants
Peroxidase
Peroxidation
Phenols
Plant cells
Plant Physiology
Plant Sciences
Polyphenol oxidase
Research Papers
Scavenging
Superoxide dismutase
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Summary:In the present study, impact of static magnetic field (SMF) on callus growth, lipid peroxidation and antioxidative defense system of Anthemis gilanica were investigated. The in vitro hypocotyl explants were placed in Murashige and Skoog medium supplemented with 0.5 mg L –1 of kinetin and 2 mg L –1 of NAA, and then exposed to different intensities of SMF (0, 2, 4, and 6 mT) for 1 h. SMF increased callus fresh and dry weights, protein, and proline content, especially at 4 and 6 mT, and decreased lipid peroxidation and H 2 O 2 levels comparing to control. Antioxidant enzyme activities including peroxidase, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase, and polyphenol oxidase were promoted significantly under SMF. The electrophoretic pattern of CAT and SOD isozymes showed that the intensity of isoform bands increased under SMF and the isoforms of Cu/Zn–SOD and CAT3 displayed the main role in SOD and CAT activities to scavenge the free radicals. Also, SMF increased significantly total phenol, flavonoid, flavonol, and anthocyanin contents and following DPPH scavenging activity were promoted. It seems that SMF can be used as an efficient elicitor for the supportable production of biomass and bioactive compounds in medicinal plant cells.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443722040124