Antimicrobial efficacy of Andrographis paniculata, sodium hypochlorite, and their combination with diode laser on endodontic pathogen-Enterococcus faecalis-An in vitro study

Aim: To evaluate and compare the antimicrobial efficacy of Andrographis paniculata and sodium hypochlorite with diode laser on endodontic pathogen Enterococcus faecalis. Materials and Methods: An in vitro study in which 40 freshly extracted single rooted anterior teeth with no root caries, apical fr...

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Bibliographic Details
Published in:Journal of international oral health 2022-05, Vol.14 (3), p.260-265
Main Authors: Malavika, K, Sekar, Rajakumar, Ramar, Kavitha
Format: Article
Language:English
Subjects:
Andrographis paniculata
Care and treatment
Comparative analysis
Complications and side effects
Dental caries
Disinfection
Enterococcal infections
Enterococcus faecalis
Inoculum
Lasers
Methods
Pathogens
Root canal therapy
Semiconductor lasers
Sodium
Sodium hypochlorite
Statistical analysis
Teeth
Testing
Variance analysis
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Summary:Aim: To evaluate and compare the antimicrobial efficacy of Andrographis paniculata and sodium hypochlorite with diode laser on endodontic pathogen Enterococcus faecalis. Materials and Methods: An in vitro study in which 40 freshly extracted single rooted anterior teeth with no root caries, apical fractures are collected. A sample size was calculated using GFNx01Power Software. All the teeth are decoronated and prepared by using ProTaper Gold (DENTSPLY) rotary file system. The apex is then sealed and waterproofed by applying nail varnish. Precultured inoculum of E. faecalis strain is inoculated and then teeth are randomly divided into four groups with 10 teeth in each group; each group is irrigated with 0.5% of A. paniculata, 3% of sodium hypochlorite, laser-activated 3% sodium hypochlorite, and laser-activated 0.5% A. paniculata, respectively. Pre- and postirrigation colonial count is obtained. Statistical analysis of all the data was analyzed using SPSS 20.0 (Armonk, NY, USA); Analysis of Variance (ANOVA) was performed for comparing variation between groups and multiple comparison tests performed for intergroup comparison. Results: The reduction of colony count postirrigation of about 96% in group 4, 90% in group 3, 48% in group 1 with a least reduction of about 24% in group 2 is observed (P = 0.000). Conclusion: All four irrigation methods included in this study proved to be effective against E. faecalis, but A. paniculata with diode laser came off as an equally efficient method of disinfection compared to sodium hypochlorite with diode laser combination and highly efficient than plain A. paniculata and sodium hypochlorite.
ISSN:0976-7428
0976-1799
DOI:10.4103/jioh.jioh_39_22