Loading…

Experimental pancreatitis results in increased blood-brain barrier permeability in rats: A potential role of MCP-1

OBJECTIVE:  To measure the changes of blood‐brain barrier (BBB) permeability in rats with acute pancreatitis (AP) and to investigate the role of monocyte chemoattractant protein (MCP)‐1 expression in this alteration. METHODS:  Rat model of severe acute pancreatitis (SAP) and mild acute pancreatitis...

Full description

Saved in:
Bibliographic Details
Published in:Journal of digestive diseases 2012-03, Vol.13 (3), p.179-185
Main Authors: DING, Zhen, LIU, Jun, LIN, Rong, HOU, Xiao Hua
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c4838-65dd5cbde96d6dd9f05477baaf55efb07139b0fad2fbed943e0e106a8012cc933
cites cdi_FETCH-LOGICAL-c4838-65dd5cbde96d6dd9f05477baaf55efb07139b0fad2fbed943e0e106a8012cc933
container_end_page 185
container_issue 3
container_start_page 179
container_title Journal of digestive diseases
container_volume 13
creator DING, Zhen
LIU, Jun
LIN, Rong
HOU, Xiao Hua
description OBJECTIVE:  To measure the changes of blood‐brain barrier (BBB) permeability in rats with acute pancreatitis (AP) and to investigate the role of monocyte chemoattractant protein (MCP)‐1 expression in this alteration. METHODS:  Rat model of severe acute pancreatitis (SAP) and mild acute pancreatitis (MAP) was induced by pancreatic duct infusion of 5% and 0.5% sodium choleate, respectively, and a saline infusion was used in the control. The severity of AP was evaluated by a pathological score system. BBB permeability was detected by Evan's blue tracer and BBB tight junction was assessed by brain occludin expression. Immunohistochemistry and real‐time polymerase chain reaction were used to detect MCP‐1 expression in the brain. Nifedipine was used as the antagonist of MCP‐1. RESULTS:  Compared to the control group, change of BBB permeability was more significant in SAP groups, but not in MAP groups. Occludin level decreased 12 h after SAP induction. Pathological score of SAP group was higher than that in MAP group. BBB opening was associated with pancreatic injury. Brain MCP‐1 expression was detected in all the SAP groups, which was correlated with increased BBB permeability, but was not found in the control group or the MAP group. After treatment with nifedipine, brain MCP‐1 level decreased and BBB function improved synchronously in SAP groups. CONCLUSIONS:  BBB permeability increased in SAP significantly and time‐dependently, and was correlated with brain MCP‐1 expression. Nifedipine may improve BBB function by inhibiting MCP‐1 expression.
doi_str_mv 10.1111/j.1751-2980.2011.00568.x
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2688334049</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2688334049</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4838-65dd5cbde96d6dd9f05477baaf55efb07139b0fad2fbed943e0e106a8012cc933</originalsourceid><addsrcrecordid>eNqNkFFr2zAUhcXYaLquf2EI9mxXsixZLnspTpsVuraMjcFehGRdg1In9iSFJv--cpPmueKCLveecy58CGFKcprexTKnFadZUUuSF4TSnBAuZL79gE6Pi4_Hvipm6HMIy0lUSXGCZkXBuGCUnSJ_vR3BuxWso-7xqNetBx1ddAF7CJs-BuzWqaZxAItNPww2M16nqdHeO_A4BaxAG9e7uJvUXsdwia_wOMQU61KuH3rAQ4d_No8Z_YI-dboPcH74z9Cfm-vfzY_s7mFx21zdZW0pmcwEt5a3xkItrLC27ggvq8po3XEOnSEVZbUhnbZFZ8DWJQMClAgtCS3atmbsDH3b545--L-BENVy2Ph1OqkKISVjJSnrpJJ7VeuHEDx0akw4tN8pStQEWy3VxFFNTNUEW73CVttk_Xo4sDErsEfjG90k-L4XPLsedu8OVs18nppkz_Z2FyJsj3btn5SoWMXV3_uF-rcoH39x2qgb9gKlqJ3N</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2688334049</pqid></control><display><type>article</type><title>Experimental pancreatitis results in increased blood-brain barrier permeability in rats: A potential role of MCP-1</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>DING, Zhen ; LIU, Jun ; LIN, Rong ; HOU, Xiao Hua</creator><creatorcontrib>DING, Zhen ; LIU, Jun ; LIN, Rong ; HOU, Xiao Hua</creatorcontrib><description>OBJECTIVE:  To measure the changes of blood‐brain barrier (BBB) permeability in rats with acute pancreatitis (AP) and to investigate the role of monocyte chemoattractant protein (MCP)‐1 expression in this alteration. METHODS:  Rat model of severe acute pancreatitis (SAP) and mild acute pancreatitis (MAP) was induced by pancreatic duct infusion of 5% and 0.5% sodium choleate, respectively, and a saline infusion was used in the control. The severity of AP was evaluated by a pathological score system. BBB permeability was detected by Evan's blue tracer and BBB tight junction was assessed by brain occludin expression. Immunohistochemistry and real‐time polymerase chain reaction were used to detect MCP‐1 expression in the brain. Nifedipine was used as the antagonist of MCP‐1. RESULTS:  Compared to the control group, change of BBB permeability was more significant in SAP groups, but not in MAP groups. Occludin level decreased 12 h after SAP induction. Pathological score of SAP group was higher than that in MAP group. BBB opening was associated with pancreatic injury. Brain MCP‐1 expression was detected in all the SAP groups, which was correlated with increased BBB permeability, but was not found in the control group or the MAP group. After treatment with nifedipine, brain MCP‐1 level decreased and BBB function improved synchronously in SAP groups. CONCLUSIONS:  BBB permeability increased in SAP significantly and time‐dependently, and was correlated with brain MCP‐1 expression. Nifedipine may improve BBB function by inhibiting MCP‐1 expression.</description><identifier>ISSN: 1751-2972</identifier><identifier>EISSN: 1751-2980</identifier><identifier>DOI: 10.1111/j.1751-2980.2011.00568.x</identifier><identifier>PMID: 22356313</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Acute Disease ; acute pancreatitis ; Animals ; Blood-brain barrier ; Blood-Brain Barrier - metabolism ; Blood-Brain Barrier - physiopathology ; Brain injury ; Calcium Channel Blockers - pharmacology ; Chemokine CCL2 - drug effects ; Chemokine CCL2 - metabolism ; Evans Blue - pharmacokinetics ; Immunohistochemistry ; Male ; Membrane permeability ; Membrane Proteins - metabolism ; Monocyte chemoattractant protein ; monocyte chemoattractant protein-1 ; Monocytes ; Nifedipine ; Nifedipine - pharmacology ; Occludin ; Pancreas ; Pancreatitis ; Pancreatitis - chemically induced ; Pancreatitis - pathology ; Pancreatitis - physiopathology ; Permeability ; Permeability - drug effects ; Rats ; Rats, Wistar ; Severity of Illness Index ; Sodium Cholate ; Up-Regulation</subject><ispartof>Journal of digestive diseases, 2012-03, Vol.13 (3), p.179-185</ispartof><rights>2012 The Authors. Journal of Digestive Diseases © 2012 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Blackwell Publishing Asia Pty Ltd.</rights><rights>Copyright Wiley Subscription Services, Inc. Mar 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4838-65dd5cbde96d6dd9f05477baaf55efb07139b0fad2fbed943e0e106a8012cc933</citedby><cites>FETCH-LOGICAL-c4838-65dd5cbde96d6dd9f05477baaf55efb07139b0fad2fbed943e0e106a8012cc933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22356313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DING, Zhen</creatorcontrib><creatorcontrib>LIU, Jun</creatorcontrib><creatorcontrib>LIN, Rong</creatorcontrib><creatorcontrib>HOU, Xiao Hua</creatorcontrib><title>Experimental pancreatitis results in increased blood-brain barrier permeability in rats: A potential role of MCP-1</title><title>Journal of digestive diseases</title><addtitle>J Dig Dis</addtitle><description>OBJECTIVE:  To measure the changes of blood‐brain barrier (BBB) permeability in rats with acute pancreatitis (AP) and to investigate the role of monocyte chemoattractant protein (MCP)‐1 expression in this alteration. METHODS:  Rat model of severe acute pancreatitis (SAP) and mild acute pancreatitis (MAP) was induced by pancreatic duct infusion of 5% and 0.5% sodium choleate, respectively, and a saline infusion was used in the control. The severity of AP was evaluated by a pathological score system. BBB permeability was detected by Evan's blue tracer and BBB tight junction was assessed by brain occludin expression. Immunohistochemistry and real‐time polymerase chain reaction were used to detect MCP‐1 expression in the brain. Nifedipine was used as the antagonist of MCP‐1. RESULTS:  Compared to the control group, change of BBB permeability was more significant in SAP groups, but not in MAP groups. Occludin level decreased 12 h after SAP induction. Pathological score of SAP group was higher than that in MAP group. BBB opening was associated with pancreatic injury. Brain MCP‐1 expression was detected in all the SAP groups, which was correlated with increased BBB permeability, but was not found in the control group or the MAP group. After treatment with nifedipine, brain MCP‐1 level decreased and BBB function improved synchronously in SAP groups. CONCLUSIONS:  BBB permeability increased in SAP significantly and time‐dependently, and was correlated with brain MCP‐1 expression. Nifedipine may improve BBB function by inhibiting MCP‐1 expression.</description><subject>Acute Disease</subject><subject>acute pancreatitis</subject><subject>Animals</subject><subject>Blood-brain barrier</subject><subject>Blood-Brain Barrier - metabolism</subject><subject>Blood-Brain Barrier - physiopathology</subject><subject>Brain injury</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Chemokine CCL2 - drug effects</subject><subject>Chemokine CCL2 - metabolism</subject><subject>Evans Blue - pharmacokinetics</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Membrane permeability</subject><subject>Membrane Proteins - metabolism</subject><subject>Monocyte chemoattractant protein</subject><subject>monocyte chemoattractant protein-1</subject><subject>Monocytes</subject><subject>Nifedipine</subject><subject>Nifedipine - pharmacology</subject><subject>Occludin</subject><subject>Pancreas</subject><subject>Pancreatitis</subject><subject>Pancreatitis - chemically induced</subject><subject>Pancreatitis - pathology</subject><subject>Pancreatitis - physiopathology</subject><subject>Permeability</subject><subject>Permeability - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Severity of Illness Index</subject><subject>Sodium Cholate</subject><subject>Up-Regulation</subject><issn>1751-2972</issn><issn>1751-2980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkFFr2zAUhcXYaLquf2EI9mxXsixZLnspTpsVuraMjcFehGRdg1In9iSFJv--cpPmueKCLveecy58CGFKcprexTKnFadZUUuSF4TSnBAuZL79gE6Pi4_Hvipm6HMIy0lUSXGCZkXBuGCUnSJ_vR3BuxWso-7xqNetBx1ddAF7CJs-BuzWqaZxAItNPww2M16nqdHeO_A4BaxAG9e7uJvUXsdwia_wOMQU61KuH3rAQ4d_No8Z_YI-dboPcH74z9Cfm-vfzY_s7mFx21zdZW0pmcwEt5a3xkItrLC27ggvq8po3XEOnSEVZbUhnbZFZ8DWJQMClAgtCS3atmbsDH3b545--L-BENVy2Ph1OqkKISVjJSnrpJJ7VeuHEDx0akw4tN8pStQEWy3VxFFNTNUEW73CVttk_Xo4sDErsEfjG90k-L4XPLsedu8OVs18nppkz_Z2FyJsj3btn5SoWMXV3_uF-rcoH39x2qgb9gKlqJ3N</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>DING, Zhen</creator><creator>LIU, Jun</creator><creator>LIN, Rong</creator><creator>HOU, Xiao Hua</creator><general>Blackwell Publishing Asia</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope></search><sort><creationdate>201203</creationdate><title>Experimental pancreatitis results in increased blood-brain barrier permeability in rats: A potential role of MCP-1</title><author>DING, Zhen ; LIU, Jun ; LIN, Rong ; HOU, Xiao Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4838-65dd5cbde96d6dd9f05477baaf55efb07139b0fad2fbed943e0e106a8012cc933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acute Disease</topic><topic>acute pancreatitis</topic><topic>Animals</topic><topic>Blood-brain barrier</topic><topic>Blood-Brain Barrier - metabolism</topic><topic>Blood-Brain Barrier - physiopathology</topic><topic>Brain injury</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Chemokine CCL2 - drug effects</topic><topic>Chemokine CCL2 - metabolism</topic><topic>Evans Blue - pharmacokinetics</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Membrane permeability</topic><topic>Membrane Proteins - metabolism</topic><topic>Monocyte chemoattractant protein</topic><topic>monocyte chemoattractant protein-1</topic><topic>Monocytes</topic><topic>Nifedipine</topic><topic>Nifedipine - pharmacology</topic><topic>Occludin</topic><topic>Pancreas</topic><topic>Pancreatitis</topic><topic>Pancreatitis - chemically induced</topic><topic>Pancreatitis - pathology</topic><topic>Pancreatitis - physiopathology</topic><topic>Permeability</topic><topic>Permeability - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Severity of Illness Index</topic><topic>Sodium Cholate</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DING, Zhen</creatorcontrib><creatorcontrib>LIU, Jun</creatorcontrib><creatorcontrib>LIN, Rong</creatorcontrib><creatorcontrib>HOU, Xiao Hua</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><jtitle>Journal of digestive diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DING, Zhen</au><au>LIU, Jun</au><au>LIN, Rong</au><au>HOU, Xiao Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Experimental pancreatitis results in increased blood-brain barrier permeability in rats: A potential role of MCP-1</atitle><jtitle>Journal of digestive diseases</jtitle><addtitle>J Dig Dis</addtitle><date>2012-03</date><risdate>2012</risdate><volume>13</volume><issue>3</issue><spage>179</spage><epage>185</epage><pages>179-185</pages><issn>1751-2972</issn><eissn>1751-2980</eissn><abstract>OBJECTIVE:  To measure the changes of blood‐brain barrier (BBB) permeability in rats with acute pancreatitis (AP) and to investigate the role of monocyte chemoattractant protein (MCP)‐1 expression in this alteration. METHODS:  Rat model of severe acute pancreatitis (SAP) and mild acute pancreatitis (MAP) was induced by pancreatic duct infusion of 5% and 0.5% sodium choleate, respectively, and a saline infusion was used in the control. The severity of AP was evaluated by a pathological score system. BBB permeability was detected by Evan's blue tracer and BBB tight junction was assessed by brain occludin expression. Immunohistochemistry and real‐time polymerase chain reaction were used to detect MCP‐1 expression in the brain. Nifedipine was used as the antagonist of MCP‐1. RESULTS:  Compared to the control group, change of BBB permeability was more significant in SAP groups, but not in MAP groups. Occludin level decreased 12 h after SAP induction. Pathological score of SAP group was higher than that in MAP group. BBB opening was associated with pancreatic injury. Brain MCP‐1 expression was detected in all the SAP groups, which was correlated with increased BBB permeability, but was not found in the control group or the MAP group. After treatment with nifedipine, brain MCP‐1 level decreased and BBB function improved synchronously in SAP groups. CONCLUSIONS:  BBB permeability increased in SAP significantly and time‐dependently, and was correlated with brain MCP‐1 expression. Nifedipine may improve BBB function by inhibiting MCP‐1 expression.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>22356313</pmid><doi>10.1111/j.1751-2980.2011.00568.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1751-2972
ispartof Journal of digestive diseases, 2012-03, Vol.13 (3), p.179-185
issn 1751-2972
1751-2980
language eng
recordid cdi_proquest_journals_2688334049
source Wiley-Blackwell Read & Publish Collection
subjects Acute Disease
acute pancreatitis
Animals
Blood-brain barrier
Blood-Brain Barrier - metabolism
Blood-Brain Barrier - physiopathology
Brain injury
Calcium Channel Blockers - pharmacology
Chemokine CCL2 - drug effects
Chemokine CCL2 - metabolism
Evans Blue - pharmacokinetics
Immunohistochemistry
Male
Membrane permeability
Membrane Proteins - metabolism
Monocyte chemoattractant protein
monocyte chemoattractant protein-1
Monocytes
Nifedipine
Nifedipine - pharmacology
Occludin
Pancreas
Pancreatitis
Pancreatitis - chemically induced
Pancreatitis - pathology
Pancreatitis - physiopathology
Permeability
Permeability - drug effects
Rats
Rats, Wistar
Severity of Illness Index
Sodium Cholate
Up-Regulation
title Experimental pancreatitis results in increased blood-brain barrier permeability in rats: A potential role of MCP-1
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T16%3A20%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Experimental%20pancreatitis%20results%20in%20increased%20blood-brain%20barrier%20permeability%20in%20rats:%20A%20potential%20role%20of%20MCP-1&rft.jtitle=Journal%20of%20digestive%20diseases&rft.au=DING,%20Zhen&rft.date=2012-03&rft.volume=13&rft.issue=3&rft.spage=179&rft.epage=185&rft.pages=179-185&rft.issn=1751-2972&rft.eissn=1751-2980&rft_id=info:doi/10.1111/j.1751-2980.2011.00568.x&rft_dat=%3Cproquest_cross%3E2688334049%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4838-65dd5cbde96d6dd9f05477baaf55efb07139b0fad2fbed943e0e106a8012cc933%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2688334049&rft_id=info:pmid/22356313&rfr_iscdi=true