Development of a fluorescent nanoprobe based on an amphiphilic single-benzene-based fluorophore for lipid droplet detection and its practical applications

Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualizat...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2022-07, Vol.2 (27), p.5423-5433
Main Authors: Jung, Yuna, Jin, Ji Hye, Kim, Youngseo, Oh, Ji Hyeon, Moon, Heechang, Jeong, Huisu, Kim, Jaehoon, Park, Yoon Kyung, Oh, Yohan, Park, Sungnam, Kim, Dokyoung
Format: Article
Language:English
Subjects:
Aqueous solutions
Benzene
Biocompatibility
Droplets
Fluorescence
Fluorescent indicators
Hydrocarbons
Hydrophobicity
Industrial applications
Lipids
Medical research
Metabolic pathways
Micelles
Organelles
Stem cells
Visualization
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Summary:Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualization using fluorescent probes has gained attention. Herein, a new fluorescent nanoprobe, BMeS-Ali , is developed that can sense LDs based on an amphiphilic single benzene-based fluorophore (SBBF). BMeS-Ali consists of hydrophilic (NH 2 ) and hydrophobic (C 12 H 25 ) moieties and exists as a micelle nanostructure in aqueous media. BMeS-Ali has a weak fluorescence, but its emission was dramatically enhanced upon exposure to the LD components such as oleic acids (OA) by reassembling its nano-formulation. BMeS-Ali showed a selective LD staining ability and great biocompatibility in cells (cancer cells and stem cells). It also showed a practical sensing ability towards biologically derived lipids and can be applied to the visualization of human fingerprints. We found that the nanoprobe BMeS-Ali has significant potential to serve as a practical dye and sensor for lipids, especially for LD imaging in the biomedical research area and broader industrial applications. A new fluorescent nanoprobe based on a single-benzene-based fluorophore is developed for the detection and bioimaging of lipid droplets and applied in the visualization of fingerprints.
ISSN:1477-0520
1477-0539
DOI:10.1039/d2ob00830k