Development of paper-based aptasensor for circulating tumor cells detection in the breast cancer

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2022-04, Vol.910, p.116182, Article 116182
• Main Authors: Khoshroo, Alireza, Fattahi, Ali, Hosseinzadeh, Laleh
• Format: Article
• Language: English
• Subjects: Aptasensor; Breast cancer; Cancer cell; Circulating tumor cells; Electrochemical impedance spectroscopy; Electrodes; MCF-7 cells; Metastasis; Paper-based sensor; Tumors; Voltammetry
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2022.116182

[Display omitted] •A reliable diagnostic test presents based on the paper based system.•The three electrode system constructed on paper as platform.•The system shows sensitive behavior for MCF-7 cell in serum samples. Rapid diagnosis of circulating tumor cells (CTC) is of great importance towards patients’ treatment. In this work, a simple paper-based sensing platform for label-free detection of circulating tumor cells in the breast cancer cells (MCF-7) is reported. This paper-based aptasensor (P-apt) consists of three-electrode system which constructed based on the manual screen-printed process through a patterned sticker on the paper. To manufacture the MCF-7 sensor, the working electrode of P-apt was functionalized with aptamers that are selective to mucin 1 proteins on the surface of MCF-7 cells. The performance of the prepared P-apt was investigated with cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The linear range was between 20 and 1×106 cell mL−1, and the detection limit was 7 cell mL−1. Furthermore, the P-apt was able to detect MCF-7 cells in serum samples. The prepared P-apt with simple manufacturing process can potentially be developed to detect various circulating tumor cells. In conclusion, presented P-apt as a low-cost, rapid and robust sensor can be use to detect breast cancer metastases and follow up of metastases treatment.