Protein Kinase Inhibition, Antibacterial Activity, and Characterization of Phytoextract-Mediated Silver Nanoparticles Using Aqueous Extracts of Ifloga spicata

• Published in: Journal of nanomaterials 2022, Vol.2022 (1)
• Main Authors: Gul, Ahmad, Wahab, Abdul, Fozia, Fozia, Shah, Syed Majid, Gul, Rukhsana, Ali, Jamshed, Khan, Nauman Rahim, Ahmad, Ijaz, Ahmad, Nisar, Zia, Muhammad, Ullah, Riaz, Alotaibi, Amal, Sultan, Mujeeb A.
• Format: Article
• Language: English
• Subjects: Alkanes; Alzheimer's disease; Amides; Amines; Bacterial infections; Biomolecules; Carbonyls; Chemicals; Drug delivery systems; Fourier transforms; Functional groups; Infrared analysis; Infrared spectroscopy; Kinases; Morphology; Nanomaterials; Nanoparticles; Phenols; Phosphorylation; Proteins; Reducing agents; Scanning electron microscopy; Signal transduction; Silver; Spectrum analysis; Stability analysis; Stretching; X-ray diffraction
• ISSN: 1687-4110; 1687-4129
• DOI: 10.1155/2022/4447857

This research work was focused on green synthesis of silver nanoparticles (Ag-NPs) employing an aqueous plant extract of Ifloga spicata. The extract of plant used in the silver nanoparticle synthesis served as a stabilizing and reducing agent. The silver nanoparticles were characterized via various techniques such as X-ray diffraction (XRD) technique, scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, and UV-visible spectroscopy. The FT-IR analysis showed an identification of functional groups of biomolecules present in plant extract like O-H stretching of phenolic compounds and the C-H stretch of alkanes, C=O is stretching vibration of carbonyl, C-N is stretching of amines and amides, and the C-O is stretching of ester that facilitates the formation of Ag-NPs. The spherical geometry and crystalline morphology with an average crystal size of 15-28 nm of Ag-NPs were revealed using SEM and XRD analysis, respectively. The stability of Ag-NPs was checked through changing the pH and temperature of the reaction mixture. It was examined that synthesized Ag-NPs were stable at 60°C temperatures and nearly neutral pH 8. The synthesized Ag-NPs also exhibit potential antibacterial activity that were investigated against a selected bacterial pathogens, like E. aerogens, B. bronchiseptica, and S. typhimurium. The silver nanoparticles exhibited a maximum zone of inhibition of 18.1±1.73 mm, 14.3±1.18 mm, and 19.5±1.30 mm against E. aerogens, B. bronchiseptica, S. typhimurium, respectively. The synthesized Ag-NPs exhibit promising protein kinase inhibition at 200 (μg/mL) as compared to plant extracts.