Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)

ABSTRACT Arthropods have progressed with efficacious approaches to utilize plant cellulose for energy development and its study would be of a great importance in the field of bioenergy and integrated pest management. The study was aimed to evaluate cellulolytic activity which was detected in the gut...

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Bibliographic Details
Published in:Pakistan journal of zoology 2022-10, Vol.54 (5), p.2061
Main Authors: Sohail, Mubasshir, Soomro, Qadeer Ahmed, Muhammad, Raza, Asif, Muhammad Usman, Rauf, Imran
Format: Article
Language:English
Subjects:
Agricultural practices
Arthropods
Biochemistry
Carboxymethylcellulose
Cations
Cellulase
Cellulose
Chemical properties
Chromatography
Divalent cations
Electrophoresis
Endoglucanase
Enzymatic activity
Enzyme activity
Enzymes
Ethylenediaminetetraacetic acids
Gel chromatography
Gel electrophoresis
Gel filtration
Integrated pest management
Mangoes
Mealy bugs
Molecular weight
Optimization
Pest control
Plant extracts
Proteins
Sodium
Sodium lauryl sulfate
Substrates
Sugar
Thermal stability
Variance analysis
Zoological research
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Summary:ABSTRACT Arthropods have progressed with efficacious approaches to utilize plant cellulose for energy development and its study would be of a great importance in the field of bioenergy and integrated pest management. The study was aimed to evaluate cellulolytic activity which was detected in the gut of mango mealybug, (Drosicha stebbingi). Initially, cellulolytic activity was detected from crude proteins using substrate-agar plate assay and laterally confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancing and inhibitory effect upon different concentrations. Effect of inhibitors (β-mercaptoethanol, EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa.
ISSN:0030-9923
0030-9923
DOI:10.17582/journal.pjz/20201118071114