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Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)
ABSTRACT Arthropods have progressed with efficacious approaches to utilize plant cellulose for energy development and its study would be of a great importance in the field of bioenergy and integrated pest management. The study was aimed to evaluate cellulolytic activity which was detected in the gut...
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| Published in: | Pakistan journal of zoology 2022-10, Vol.54 (5), p.2061 |
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| Language: | English |
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| container_issue | 5 |
| container_start_page | 2061 |
| container_title | Pakistan journal of zoology |
| container_volume | 54 |
| creator | Sohail, Mubasshir Soomro, Qadeer Ahmed Muhammad, Raza Asif, Muhammad Usman Rauf, Imran |
| description | ABSTRACT Arthropods have progressed with efficacious approaches to utilize plant cellulose for energy development and its study would be of a great importance in the field of bioenergy and integrated pest management. The study was aimed to evaluate cellulolytic activity which was detected in the gut of mango mealybug, (Drosicha stebbingi). Initially, cellulolytic activity was detected from crude proteins using substrate-agar plate assay and laterally confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancing and inhibitory effect upon different concentrations. Effect of inhibitors (β-mercaptoethanol, EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa. |
| doi_str_mv | 10.17582/journal.pjz/20201118071114 |
| format | article |
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The study was aimed to evaluate cellulolytic activity which was detected in the gut of mango mealybug, (Drosicha stebbingi). Initially, cellulolytic activity was detected from crude proteins using substrate-agar plate assay and laterally confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancing and inhibitory effect upon different concentrations. Effect of inhibitors (β-mercaptoethanol, EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa.</description><identifier>ISSN: 0030-9923</identifier><identifier>EISSN: 0030-9923</identifier><identifier>DOI: 10.17582/journal.pjz/20201118071114</identifier><language>eng</language><publisher>Lahore: Knowledge Bylanes</publisher><subject>Agricultural practices ; Arthropods ; Biochemistry ; Carboxymethylcellulose ; Cations ; Cellulase ; Cellulose ; Chemical properties ; Chromatography ; Divalent cations ; Electrophoresis ; Endoglucanase ; Enzymatic activity ; Enzyme activity ; Enzymes ; Ethylenediaminetetraacetic acids ; Gel chromatography ; Gel electrophoresis ; Gel filtration ; Integrated pest management ; Mangoes ; Mealy bugs ; Molecular weight ; Optimization ; Pest control ; Plant extracts ; Proteins ; Sodium ; Sodium lauryl sulfate ; Substrates ; Sugar ; Thermal stability ; Variance analysis ; Zoological research</subject><ispartof>Pakistan journal of zoology, 2022-10, Vol.54 (5), p.2061</ispartof><rights>COPYRIGHT 2022 Knowledge Bylanes</rights><rights>(c)2022 Pakistan Journal of Zoology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Sohail, Mubasshir</creatorcontrib><creatorcontrib>Soomro, Qadeer Ahmed</creatorcontrib><creatorcontrib>Muhammad, Raza</creatorcontrib><creatorcontrib>Asif, Muhammad Usman</creatorcontrib><creatorcontrib>Rauf, Imran</creatorcontrib><title>Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)</title><title>Pakistan journal of zoology</title><description>ABSTRACT Arthropods have progressed with efficacious approaches to utilize plant cellulose for energy development and its study would be of a great importance in the field of bioenergy and integrated pest management. The study was aimed to evaluate cellulolytic activity which was detected in the gut of mango mealybug, (Drosicha stebbingi). Initially, cellulolytic activity was detected from crude proteins using substrate-agar plate assay and laterally confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancing and inhibitory effect upon different concentrations. Effect of inhibitors (β-mercaptoethanol, EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa.</description><subject>Agricultural practices</subject><subject>Arthropods</subject><subject>Biochemistry</subject><subject>Carboxymethylcellulose</subject><subject>Cations</subject><subject>Cellulase</subject><subject>Cellulose</subject><subject>Chemical properties</subject><subject>Chromatography</subject><subject>Divalent cations</subject><subject>Electrophoresis</subject><subject>Endoglucanase</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acids</subject><subject>Gel chromatography</subject><subject>Gel electrophoresis</subject><subject>Gel filtration</subject><subject>Integrated pest management</subject><subject>Mangoes</subject><subject>Mealy bugs</subject><subject>Molecular weight</subject><subject>Optimization</subject><subject>Pest control</subject><subject>Plant extracts</subject><subject>Proteins</subject><subject>Sodium</subject><subject>Sodium lauryl sulfate</subject><subject>Substrates</subject><subject>Sugar</subject><subject>Thermal stability</subject><subject>Variance analysis</subject><subject>Zoological research</subject><issn>0030-9923</issn><issn>0030-9923</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpVUV1LHDEUHYpCxfofAr604K75mHzVJ1m_CkoL2ufhTuZmzDI7WZOZB33qTzftWtAEcsPlnnM551TVMaNLpqXhp-s4pxGG5Xb9csopp4wxQ3V560_VAaWCLqzlYu_d_3N1lPOallMrybk5qP7cu4Q4hrE_IatHSOAmTOEFphBHAmNHfs0p-OB2jejJCodhHiAj8SluyB2MfSR3CMNzO_fkIsUc3COQPGHbFtZArv_yk683cRO3hRu-F0zqIcUudIDfvlT7HoaMR2_1sPp9dfmwulnc_rz-sTq_XTiuDVs4gR11nWTaSklRoG8FF9bzVgkta7DQKq1qlFIYoevOmo4KKy23IFtepB9WxzvebYpPM-apebMvN1xZVTOtlClTy91UDwM2YfRxKpaU2-EmuDiiD6V_Xky2ymhjC-BsB3BFeU7om20KG0jPDaPNv5j-72lKTM3HmMQrbHaJdA</recordid><startdate>20221031</startdate><enddate>20221031</enddate><creator>Sohail, Mubasshir</creator><creator>Soomro, Qadeer Ahmed</creator><creator>Muhammad, Raza</creator><creator>Asif, Muhammad Usman</creator><creator>Rauf, Imran</creator><general>Knowledge Bylanes</general><general>AsiaNet Pakistan (Pvt) Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SS</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20221031</creationdate><title>Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)</title><author>Sohail, Mubasshir ; 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The study was aimed to evaluate cellulolytic activity which was detected in the gut of mango mealybug, (Drosicha stebbingi). Initially, cellulolytic activity was detected from crude proteins using substrate-agar plate assay and laterally confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancing and inhibitory effect upon different concentrations. Effect of inhibitors (β-mercaptoethanol, EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa.</abstract><cop>Lahore</cop><pub>Knowledge Bylanes</pub><doi>10.17582/journal.pjz/20201118071114</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Pakistan journal of zoology, 2022-10, Vol.54 (5), p.2061 |
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| source | Nexis UK |
| subjects | Agricultural practices Arthropods Biochemistry Carboxymethylcellulose Cations Cellulase Cellulose Chemical properties Chromatography Divalent cations Electrophoresis Endoglucanase Enzymatic activity Enzyme activity Enzymes Ethylenediaminetetraacetic acids Gel chromatography Gel electrophoresis Gel filtration Integrated pest management Mangoes Mealy bugs Molecular weight Optimization Pest control Plant extracts Proteins Sodium Sodium lauryl sulfate Substrates Sugar Thermal stability Variance analysis Zoological research |
| title | Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae) |
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