Combination of platelet bio-specific extraction and high-performance liquid chromatography with diode-array detection/liquid chromatography-mass spectrometry method for analyzing platelet-targeted compounds in rhizoma chuanxiong

Context: Platelet is an important pharmacological target as it participates in complex processes of coagulation and hemostasis. Excessive platelet aggregation is responsible for the formation of pathogenic thrombi in patients with atherothrombotic disease. Objective: To expedite the search for plate...

Full description

Saved in:
Bibliographic Details
Published in:Pharmacognosy Magazine 2022-04, Vol.18 (78), p.427-434
Main Authors: Zhang, Qian, Yang, Yuxiu, Xiao, Yao, Wang, Fenqing, Hu, Yuanjia, Xia, Zhining, Yang, Fengqing
Format: Article
Language:English
Subjects:
Analysis
Chromatography
Esters
High performance liquid chromatography
Mass spectrometry
Methods
Scientific imaging
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Context: Platelet is an important pharmacological target as it participates in complex processes of coagulation and hemostasis. Excessive platelet aggregation is responsible for the formation of pathogenic thrombi in patients with atherothrombotic disease. Objective: To expedite the search for platelet-targeted active candidates in Rhizoma Chuanxiong (root of Ligusticum chuanxiong Hort [Umbelliferae]). Materials and Methods: Rhizoma Chuanxiong ethyl acetate extract (EAE) was obtained by refluxing extraction method. A platelet bio-specific extraction combined with high-performance liquid chromatography with diode-array detection/liquid chromatography-mass spectrometry (HPLC-DAD/LC-MS) approach was employed to screen and identify the platelet-targeted compounds in Chuanxiong EAE, and the results were confirmed by in vitro antiplatelet aggregation test using turbidimetry method. Finally, HPLC-DAD analysis was employed for the quantitative determination of these hit compounds in Chuanxiong EAE. Results: A total of five hit components were tentatively detected and identified by HPLC-DAD/LC-MS, two of which, senkyunolide A and (Z)-ligustilide, were confirmed their antiplatelet activity by in vitro platelet aggregation experiment (both with IC50
ISSN:0973-1296
0976-4062
DOI:10.4103/pm.pm_390_16