Deep sequencing unravels methyl jasmonate responsive novel miRNAs in Podophyllum hexandrum

Podophyllum hexandrum —an endangered, high-altitude medicinal herb is a vital source of the therapeutically important lignan viz. podophyllotoxin (PTOX). PTOX is widely used as a precursor for semi-synthesis of anticancer drugs etoposide, teniposide and etopophos. Although next generation sequencing...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant biochemistry and biotechnology 2022-09, Vol.31 (3), p.511-523
Main Authors: Biswas, Soumi, Hazra, Saptarshi, Chattopadhyay, Sharmila
Format: Article
Language:English
Subjects:
Antineoplastic drugs
Antitumor agents
Biomedical and Life Sciences
Biosynthesis
Cell Biology
Cell culture
Encyclopedias
Etoposide
Gene sequencing
Genes
Genomes
Herbal medicine
High altitude
Libraries
Life Sciences
Lignans
Medicinal plants
Metabolites
Methyl jasmonate
Next-generation sequencing
Original Article
Plant Biochemistry
Plant growth
Plant species
Podophyllotoxin
Podophyllum hexandrum
Protein Science
Receptors
Ribonucleic acid
RNA
Secondary metabolites
Teniposide
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Podophyllum hexandrum —an endangered, high-altitude medicinal herb is a vital source of the therapeutically important lignan viz. podophyllotoxin (PTOX). PTOX is widely used as a precursor for semi-synthesis of anticancer drugs etoposide, teniposide and etopophos. Although next generation sequencing has been employed to characterize the genes involved in PTOX biosynthesis; the complete biosynthetic pathway is yet to be known. miRNAs play pivotal roles in secondary metabolites biosynthesis besides plant growth and development. Till date, several novel miRNAs have been identified in different plant species, but novel miRNAs and their regulatory functions still remains unknown in P. hexandrum . Here, two small RNA libraries were prepared from control and MeJA treated cell cultures of P. hexandrum . High-throughput sequencing of these small RNA libraries yielded 59 novel miRNAs in control and 66 novel miRNAs in treated P. hexandrum apart from known miRNAs. The identified miRNAs targeted different mRNAs. Kyoto Encyclopedia of Genes and Genomes pathway analysis unveiled the role of these miRNAs in various secondary metabolites biosynthetic pathways. qRT-PCR analysis showed lower expression of a set of miRNAs identified in MeJA treated cell culture. The current study expands our knowledge of novel miRNAs mediated secondary metabolites, especially PTOX biosynthesis, in P. hexandrum .
ISSN:0971-7811
0974-1275
DOI:10.1007/s13562-021-00698-6