Cloning and characterization of linker histone H1 gene in rohu, Labeo rohita

Linker histone H1 (LHH1) is an abundant nuclear protein that condenses chromatin to form higher-order structure. The present study reported cloning and sequencing of 942 bp of LHH1 from liver tissue of rohu, Labeo rohita, with a complete coding sequence of 792 bp of having 263 amino acids. The phylo...

Full description

Saved in:
Bibliographic Details
Published in:Animal biotechnology 2022-08, Vol.33 (4), p.745-756
Main Authors: Parida, Sonali, Mohapatra, Amruta, Das, Sweta, Kumar Sahoo, Pramoda
Format: Article
Language:English
Subjects:
Amino acids
Antibacterial activity
Chromatin
Cloning
Ectoparasites
Fertilization
Histone H1
Histones
Innate immunity
Labeo rohita
linker histone H1
Liver
Ontogeny
Phylogeny
Polyinosinic:polycytidylic acid
Proteins
qRT-PCR
Sequences
Three dimensional models
Tissues
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Linker histone H1 (LHH1) is an abundant nuclear protein that condenses chromatin to form higher-order structure. The present study reported cloning and sequencing of 942 bp of LHH1 from liver tissue of rohu, Labeo rohita, with a complete coding sequence of 792 bp of having 263 amino acids. The phylogenetic tree of L. rohita LHH1 (LrLHH1) shared maximum similarity with that of Carassius auratus. The three dimensional model and domain architecture of LrLHH1 protein was also predicted using Swiss-Prot and SMART domain software. The expression of LHH1 during ontogeny showed significantly higher transcript level in milt, unfertilized eggs and up to 3 h post-fertilization followed by a dramatic decrease thereafter. The tissue-specific expression showed constitutive expression of LrLHH1 in all examined tissues. The expression of LHH1 during different infection models, namely, bacteria (Aeromonas hydrophila); ectoparasite (Argulus siamensis) and poly I:C induction revealed modulation in the level of expression at varied time points post-exposure in the liver and anterior kidney tissues of rohu. However, a synthetic peptide derived from LHH1 sequence of rohu did not have any detectable antibacterial activity. The present study provided necessary information on the role of this protein during ontogeny and innate immunity in Indian major carp species.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2020.1832506