Colorimetric detection of RNA fragments based on associated toehold-mediated reaction and gold nanoparticles

Herein we report a reverse transcription-free, label-free and enzyme-free colorimetric method for RNA nucleic acid fragments detection. The method can conveniently determine the presence of dual gene targets and distinguish single nucleotide polymorphism by visual observation. A reverse transcriptio...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2022-08, Vol.58 (62), p.8666-8669
Main Authors: Zhang, Wantong, Song, Juanjuan, Zheng, Hongzheng, Xu, Xiaowen
Format: Article
Language:English
Subjects:
Colorimetry
Fragments
Nanoparticles
Nucleic acids
Nucleotides
Polymorphism
Ribonucleic acid
RNA
Visual observation
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Summary:Herein we report a reverse transcription-free, label-free and enzyme-free colorimetric method for RNA nucleic acid fragments detection. The method can conveniently determine the presence of dual gene targets and distinguish single nucleotide polymorphism by visual observation. A reverse transcription-free, label-free and enzyme-free colorimetric method is developed for RNA nucleic acid fragment detection. The method simultaneously responds to dual viral gene fragments and distinguishes single nucleotide polymorphisms.
ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc02389j