Biocompatibility evaluation of electrospun nanofiber PLCL/fibrinogen artificial ligament

The purpose of this study was to evaluate the safety and functionality of polylactic-caprolactone (PLCL)/fibrinogen nanofibrous membranes (P/F-Ns), and to provide theoretical support for clinical application. The surface morphology, mechanical properties, hydrophilicity and fibrinogen content of P/F...

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Published in:Sheng wu yi xue gong cheng xue za zhi 2022-01 (3), p.544
Main Authors: Guo, Jiahua, Zhang, Yu, Chen, Lizuo, Xu, Liming, Mo, Xiumei, Chen, Liang
Format: Article
Language:Chinese
Subjects:
Adhesion
Anterior cruciate ligament
Biocompatibility
Cell adhesion
Cell proliferation
Cholecystokinin
Contact angle
Evaluation
Fibrinogen
Gene expression
Genes
Hydrophilicity
Knee
Ligaments
Materials testing
Measuring instruments
Mechanical properties
Nanofibers
Repair
Scanning electron microscopy
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Summary:The purpose of this study was to evaluate the safety and functionality of polylactic-caprolactone (PLCL)/fibrinogen nanofibrous membranes (P/F-Ns), and to provide theoretical support for clinical application. The surface morphology, mechanical properties, hydrophilicity and fibrinogen content of P/F-Ns were investigated by scanning electron microscope, material testing machine, contact angle measuring instrument and microplate reader, respectively. The expression of genes related to adhesion, proliferation and ligament repair of Hig-82 cells on P/F-Ns was evaluated by CCK-8 method and real-time quantitative PCR. The results showed that with the increase of fibrinogen content, the fibrous pores on the surface of P/F-Ns increased, the mechanical properties decreased, and the hydrophilicity increased, among which P/FN-2 was the most favorable for cell adhesion and proliferation. Compared with P/FN-0 without fibrinogen as control, the genes related to cellular ligament repair on P/FN-1, P/FN-2 and P/FN-3 with fib
ISSN:1001-5515