DNA biosensor based detection for neglected tropical disease: moving towards smart diagnosis

• Published in: Sensor review 2022-08, Vol.42 (5), p.517-525
• Main Authors: Stephen, Bjorn John, Suchanti, Surabhi, Jain, Devendra, Dhaliwal, Harshdeep, Sharma, Vikram, Kaur, Ramandeep, Mishra, Rajeev, Singh, Abhijeet
• Format: Article
• Language: English
• Subjects: Antibodies; Biomarkers; Biosensors; Dengue fever; Deoxyribonucleic acid; Design modifications; Diagnosis; DNA; Electrodes; Electrons; Enzymes; Equatorial regions; Hybridization; Infections; Infectious diseases; Leprosy; Nanomaterials; Nanoparticles; Pathogens; Sensors; Serology; Tropical diseases; Viral infections; Zinc oxides
• ISSN: 0260-2288; 1758-6828; 0260-2288
• DOI: 10.1108/SR-11-2021-0429

Purpose Neglected tropical diseases (NTDs) are a set of infectious diseases that primarily affect low-income countries situated near the equator. Effective diagnostic tools hold the key to stemming the spread of these infectious diseases. However, specificity is a major concern associated with current diagnostic protocols. In this regard, electrochemical deoxyribonucleic acid (DNA) biosensors could play a crucial role, as highlighted by renewed interest in their research. The purpose of this study was to highlight the current scenario for the design and development of biosensors for the detection of NTDs related pathogens. This review highlights the different types of factors involved and the modifications used to enhance sensor properties. Design/methodology/approach The authors discuss the potential of electrochemical DNA biosensors as efficient, affordable diagnostic tools for the detection of pathogens associated with NTDs by reviewing available literature. This study discusses the biosensor components, mainly the probe selection and type of electrodes used, and their potential to improve the overall design of the biosensor. Further, this study analyses the different nanomaterials used in NTD-based electrochemical DNA biosensors and discusses how their incorporation could improve the overall sensitivity and specificity of the biosensor design. Finally, this study examines the impact such techniques could have in the future on mass screening of NTDs. Findings The findings provide an in-depth analysis of electrochemical DNA biosensors for the detection of pathogens associated with NTDs. Originality/value This review provides an update on the different types and modifications of DNA biosensors that have been designed for the diagnosis of NTD-related pathogens.