A water-soluble polymer fluorescent probe via RAFT polymerization for dynamic monitoring of cellular lipid droplet levels and zebrafish imaging

Herein, the coumarin derivative was chosen as the luminescent parent and co-polymerized with a low-toxicity, highly hydrophilic terminal hydroxy polyethylene glycol to obtain the water-soluble probe P(Cou-PEG-LD). The probe P(Cou-PEG-LD) possessed improved biocompatibility, interference resistance a...

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Bibliographic Details
Published in:New journal of chemistry 2022-08, Vol.46 (34), p.16539-16546
Main Authors: Cui, Wei-Long, Wang, Mao-Hua, Yang, Yun-Hao, Qu, Jianbo, Zhang, Haitao, Zhu, Xiuzhong, Wang, Jian-Yong
Format: Article
Language:English
Subjects:
Addition polymerization
Biocompatibility
Copolymerization
Coumarin
Droplets
Fluorescent indicators
Lipids
Medical imaging
Monitoring
Polyethylene glycol
Telemedicine
Toxicity
Water chemistry
Water soluble polymers
Zebrafish
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Summary:Herein, the coumarin derivative was chosen as the luminescent parent and co-polymerized with a low-toxicity, highly hydrophilic terminal hydroxy polyethylene glycol to obtain the water-soluble probe P(Cou-PEG-LD). The probe P(Cou-PEG-LD) possessed improved biocompatibility, interference resistance and photostability. Moreover, the probe exhibited optimal targeting properties toward lipid droplets and was successfully applied for dynamic monitoring of lipid droplet levels in HeLa cells. Interestingly, the sensitive labeling of the intracellular lipid droplet content using the probe P(Cou-PEG-LD) enabled the differentiation of starved and normal cells. The probe had also been successfully applied to zebrafish bioimaging and such specific features represented the great potential of the probe for disease prediction and treatment monitoring.
ISSN:1144-0546
1369-9261
DOI:10.1039/D2NJ03226K