High antibacterial in vitro performance of gold nanoparticles synthesized by epigallocatechin 3-gallate

In the present work, we report antibacterial activity of gold nanoparticles (AuNPs) prepared using Epigallocatechin 3-Gallate (EGCG) against S. aureus, E. faecalis, P. aeruginosa and E. coli . The synthesized AuNPs-EGCG showed optimal stability in physiological in vitro conditions and could be resus...

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Bibliographic Details
Published in:Journal of materials research 2021-01, Vol.36 (2), p.518-532
Main Authors: Avila, Sady Roberto Rodriguez, Schuenck, Gisele Pereira Diniz, Silva, Laryssa Pinheiro Costa e, Keijok, Wanderson Juvencio, Xavier, Lorena Martins, Endringer, Denise Coutinho, Oliveira, Jairo Pinto, Schuenck, Ricardo Pinto, Guimarães, Marco Cesar Cunegundes
Format: Article
Language:English
Subjects:
Applied and Technical Physics
Bacteria
Biocompatibility
Biomaterials
Biomedical materials
Cell death
Chemistry and Materials Science
E coli
Gold
Inorganic Chemistry
Materials Engineering
Materials research
Materials Science
Nanoparticles
Nanotechnology
Synthesis
Toxicity
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Summary:In the present work, we report antibacterial activity of gold nanoparticles (AuNPs) prepared using Epigallocatechin 3-Gallate (EGCG) against S. aureus, E. faecalis, P. aeruginosa and E. coli . The synthesized AuNPs-EGCG showed optimal stability in physiological in vitro conditions and could be resuspended in various solvents. In vitro antibacterial activity against Gram-positive and Gram-negative bacteria was evidenced by inhibition of viable cell growth, with MIC values varying between 15 and 120 µg mL −1 in all treated bacteria. The antibacterial effects of the nanoformulation were the result of direct interaction with the surfaces of treated bacteria, causing morphological deformations that induced lysis and cell death. Besides, in vitro cytotoxicity analyses in murine fibroblast cells (L929) and human keratinocytes (HaCaT) showed high cell viability levels after 24 h of treatment with AuNPs-EGCG, with IC 50 values of 267.55 e 293.04 µg mL −1 , respectively. Thus, in its adsorbed form in gold nanoparticles, EGCG had its antibacterial activity potentiated and was less cytotoxic. AuNPs-EGCG shows high potential for future biomedical applications as an antibacterial treatment against skin infections. Graphic abstract Schematic illustration of the synthesis of gold nanoparticles using EGCG (AuNPs-EGCG), its toxic application in gram-positive and -negative bacteria and its possible mechanism by inducing structural deformations.
ISSN:0884-2914
2044-5326
DOI:10.1557/s43578-020-00012-5