Potency of a novel synthesized Ag-eugenol nanoemulsion for treating some bacterial and fungal pathogens

• Published in: Journal of materials research 2021-04, Vol.36 (7), p.1524-1537
• Main Authors: Mosallam, Farag M., Helmy, Eman A., Bendary, Mahmoud M., El-Batal, Ahmed I.
• Format: Article
• Language: English
• Subjects: Antiinfectives and antibacterials; Applied and Technical Physics; Bacteria; Biomaterials; Chemistry and Materials Science; E coli; Inorganic Chemistry; Materials Engineering; Materials research; Materials Science; Nanoemulsions; Nanoparticles; Nanotechnology; Silver; Spectrum analysis; Synthesis; Zeta potential
• ISSN: 0884-2914; 2044-5326
• DOI: 10.1557/s43578-021-00226-1

The current research deals with the synthesis and validation of the novel Ag-eugenol nanoemulsion (O/W). The synthesized Ag-eugenol nanoemulsion was validated by DLS, TEM, FT-IR, UV–VIS spectral analysis; TEM reveals the particles an average size of 30 nm and SEM images confirm spherical morphology of particles. Zeta potential measurements, presented a negative average zeta potential of − 25 mV. EDX analysis showed typical absorption peaks at 0.25 keV and 3 keV that confirm the presence of AgNPs. Elemental mapping of Ag-eugenol nanoemulsion confirms the presence of silver, eugenol moiety and the AgNPs are nucleated by eugenol moiety. Eugenol, eugenol nanoemulsion (5 µg/ml), AgNPs (25 ppm) and Ag-eugenol nanoemulsion (12.5 ppm AgNPs: eugenol 2.5 µg/ml) solutions were tested for their antimicrobial potency against Staphylococcus aureus , Bacillus subtilis , Escherichia coli , and Proteus vulgaris . Ag-eugenol nanoemulsion was effective against both tested fungi; A. fumigatus and C. albicans with MIC 12.5:2.5 ppm:µg/ml and 6.25:1.25 ppm:µg/ml, respectively, comparing to a negative effect of eugenol nanoemulsion. Graphical abstract